your favorite gene search
powered by ingenuity
|
Life Science > Proteomics > Recombinant Protein Expression > Purification & Detection > HIS-Select® Technology > HIS-Tag Protocol
|
Product No. H9914
Prior to use
Ni magnetic beads supplied in ethanol are repeatedly exchanged with wash buffer and used at a final concentration of 10% (v/v).
Step 1: Tranfer beads to cell lysate
Add 30 µl of Ni magnetic beads to soluble cell lysate and mix for 15 min at RT.
Step 2: Remove lysate
Place 96-deep well block onto a 96-well magnet for 5 min. to separate the magnetic beads in solution. Remove the lysate and discard.
Step 3: Wash beads
Add 1 ml of wash buffer to 96-deep well block, shake for 5 min. to resspend Ni magnetic beads, place again on the 96-well magnet and remove buffer. Repeat washing step.
Step 4: Add elution buffer
Add 60 µl elution buffer and shake for 3 min. Spin plate in centrifuge to ensure that all beads come in contact with the elution buffer.
Step 5: Transfer elution proten
Place 96-deep well block on 96-well magnet to separate the magnetic beads and elution buffer. Transfer 60 µl eluted protein to a 96-well microtiter plate.