Special Offer

Standardized Cloning Vectors using SnapFast™ Technology

Traditional cloning by restriction enzyme digestion remains the most popular way to insert your gene-of-interest (GOI) into an expression vector for expression in the target cell, whether that is an insect, mammalian, or microbial cell. This cloning method is easy, reliable, and cost-conscious, but lacks standardization due to labs using a variety of different plasmid backbones.

To answer the need of standardization, Oxford Genetics uses patented SnapFast™ Technology to make genetic engineering cheaper and more efficient by providing scientists with a collection of versatile cloning vectors, and a protocol to easily move the GOI from one vector to another. The vectors can be used with any cloning system you like: traditional restriction enzyme cloning, Gibson Assembly®, LIC, GeneArt®, and InfusionHD.

The concept is simple: One plasmid, a thousand possibilities. All of the DNA plasmids are based on the same core backbone - the SnapFast vector.  All of the DNA sections or components within the SnapFast system are designed to be fully inter-changeable, so you can move your GOI from one vector to another with ease. You can express your gene with a different reporter gene, with a different tag, or in a different species by simply moving the GOI into a different vector, using the SnapFast cutting sites in one fast & easy protocol.

Our most popular cloning vectors and vector sets are listed below, but the collection gives you choice of:

  • 26 peptide tags
  • 9 reporter genes
  • >20 signal peptides
  • >40 promoters
  • 10 selection markers

Browse hundreds of options easily using our NEW SELECTION GUIDE or ENHANCED SEARCHING.

 

Plasmid Product Name Product No. Application
NEW SnapFast™ Vectors based on the most popular pFLAG Vectors
pSF-CMV-NEO-NH2-PPT-3XFLAG OGS626 CMV promoter with strong expression
pSF-CMV-NEO-NH2-3XFLAG OGS627 CMV promoter with strong expression
pSF-CMV-NEO-COOH-3XFLAG OGS629 CMV promoter with strong expression
Most Popular Bacterial Reporter Genes
pSF-OXB20-BetaGal OGS207 RecA promoter driving b-galactosidase
pSF-OXB20-Fluc OGS412 OXB20 promoter driving firefly luciferase
Most Popular Bacterial Promoters
pSF-OXB20 OGS50 OXB20 promoter (strongest expression)
pSF-OXB1 OGS553 OXB1 promoter (weakest expression)
pSF-Tac OGS501 Tac promoter (IPTG inducible expression)
pSF-LacI OGS502 LacI promoter (regulated expression)
Most Popular Mammalian Reporter Genes
pSF-MINCMV-DAGFP OGS573 Minimal CMV promoter for low expression
pSF-CMV-FMDV-daGFP OGS289 Dual promoters driving IRES daGFP
pSF-CMV-RSV-SEAP AscI OGS17 Dual promoters driving SEAP
Most Popular Mammalian Promoters
pSF-SYN1 OGS503 Synapsin 1 neuron-specific promoter
pSF-CAG-AMP OGS504 CAG promoter strong and stable expression
pSF-CMV-AMP OGS2 CMV promoter strongest expression
pSF-CAG-UB-PURO OGS600 CAG promoter strong and stable expression
pSF-CMV-Kan OGS10 CMV promoter (strongest expression)
pSF-CAG-Kan OGS505 Synthetic CAG promoter (strong and stable expression)
pSF-EF1 Alpha OGS43 EF1a promoter (moderate but stable expression)
Most Popular Mammalian Selection Markers
pSF-CMV-PGK-Puro OGS394 Dual promoter for expressing two genes (puromycin)
pSF-CMV-FMDV-Hygro OGS292 Dual promoter expressing one gene (hygromycin)
pSF-CMV-Blast OGS588 CMV promoter (blasticidin)
pSF-CMV-Ub-Neo/G418 AscI OGS22 Dual promoter expressing one gene (G418)
Most Popular Mammalian Protein Tags
pSF-​CMV-​PURO-​NH2-​GST-​TEV OGS1129 N-term GST tag & TEV cleavage tag
pSF-​CMV-​PURO-​COOH-​TEV-​GST OGS1130 C-term GST tag & TEV cleavage tag
pSF-​CMV-​PURO-​NH2-​CMYC OGS3214 N-term C-myc tag
pSF-CMV-Puro-COOH-TEV-FLAG®-6His OGS1232 C-term FLAG®, 6His affinity tags & TEV cleavage tag
Most Popular Bacterial Protein Tags
pSF-OXB20-NH2-10His-EKT OGS2806 N-term 10His affinity tag & EKT cleavage tag
pSF-OXB20-NH2-FLAG(R)-6His-EKT OGS2828 N-term FLAG®, 6His affinity tags & EKT cleavage tag
pSF-OXB20-COOH-TEV-10His OGS2767 C-term 10His affinity tag & TEV cleavage tag
pSF-OXB20-COOH-TEV-FLAG(R)-6His OGS2891 C-term FLAG®, 6His affinity tags & TEV cleavage tag
Most Popular Yeast Protein Tags
pSF-TEF1-NH2-10His-EKT OGS1649 N-term 10His affinity tag & EKT cleavage tag
pSF-TEF1-NH2-FLAG®-6His-EKT OGS1658 N-term FLAG®, 6His affinity tags & EKT cleavage tag
pSF-TEF1-COOH-TEV-10His OGS1913 C-term 10His affinity tag & TEV cleavage tag
pSF-TEF1-COOH-TEV-FLAG®-6His OGS1969 C-term FLAG®, 6His affinity tags & TEV cleavage tag
Other Popular Plasmids
pSF-CMV-CRE OGS591 CRE recombinase for LoxP excision
PUC19 OGS590 industry standard high copy number cloning vector
PBR322 OGS589 industry standard low copy number cloning vector
pSF-RENILLA-PHOTINUS OGS595 bidrectional expression of two luciferase reporters
pSF-CMV-HUIGG1 HC OGS527 seamless fusion of variable antibody fragments
pSF-TEFI-TPI1-Fluc-URA3 OGS545 Yeast promoter driving firefly luciferase (plus URA3 selection)
pSF-CMV-CMV-SbfI-Ub-Puro OGS597 Dual CMV promoters for expressing two genes
Popular Plasmid Sets
Mammalian Signal Peptide Pack PP2379 Eight plasmids to optimize signal peptide use
Yeast Promoter Vector Pack PP2357 Five plasmids to optimize expression based on promoter strength
GFP Vector Pack PP2368 Five plasmids to optimize the configuration of the GFP reporter gene
Mammalian Promoters Pack PP2356 Six plasmids to optimize expression based on promoter strength

 

GeneArt is owned by Life Technologies.
Gibson Assembly is owned by Synthetic Genomics.
SnapFast is owned by Oxford Genetics.