Hematopoietic Stem Cell Culture

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Hematopoietic stem cells (HSCs) give rise to all blood cell types including myeloid and lymphoid lineages. Myeloid cells include monocytes, macrophages, neutrophils, basophils, eosinophils, erythrocytes, dendritic cells, and megakaryocytes or platelets. Lymphoid cells include T cells, B cells, and natural killer cells. HSCs are found in the bone marrow of adults, umbilical cord blood, placenta, and mobilized peripheral blood. Recent advances in hematopoietic stem cell biology have resulted in the use of HSC transplants in the treatment of cancers and other immune system disorders.

Through the process of hematopoiesis, all cellular blood components are derived from hematopoietic stem cells.

 

MilliporeSigma offers a broad range of tools and technologies to culture hematopoietic stem cells including Stemline® optimized serum-free expansion media, growth factors, cytokines and a broad range or HSC related antibodies and characterization kits.

 

 Hematopoietic Stem Cell Media and Cytokines

Stemline® II Hematopoietic Stem Cell

Developed to promote the optimal expansion of human hematopoietic stem cells (HSC) from bone marrow, mobilized peripheral blood, and cord blood, Stemline Hematopoietic Stem Cell Expansion Medium demonstrates higher total nucleated cell (TNC) fold increases than other commercially available serum-free media formulations.

Features and Benefits

  • Serum-free formulation
  • Enhanced expansion from cord blood CD34+ cells
  • Expands cells from all appropriate hematopoietic lineages in a colony-forming unit.
  • Tested extensively in 7-day and 14-day growth assays.
  • Produced in a GMP state-of-the-art facility with an available Device Master File (DMF).

Published in over 1000 Publications Here

 

 

Cytokines and Growth Factors

Browse over 2000 Cytokines and Growth Factors Here

Hematopoietic Stem Cell Growth Factors and Cytokines Here

 

HumanKine Xeno-Free Growth Factors and Cytokines

Sigma-Aldrich is proud to bring you HumanKine® growth factors and cytokines. These highly purified reagents are developed from an efficient human cell-based technology, and are excellent choices for your critical inflammation, cancer, stem cell, and antibody development research applications. 

HumanKine Growth Factors are: 

  • Produced in HEK293 cells
  • Recombinant, animal component-free
  • Have authentic human glycosylation and  post-translational modification patterns
  • Have higher biological activity vs. E.coli derived recombinant proteins
  • Highly pure, low endotoxin proteins

 

 Hematopoietic Stem Cell Characterization

Hematopoietic Stem Cell Antibodies

Popular Hematopoietic Stem Cell Antibodies
 

CD34 CD38
CD90 CD133
CD105 CD45 c-Kit SCA-1 Stem Cell Factor ALDH

AldeRed ALDH Detection Assay

Hematopoietic stem cell express elevated levels of aldehyde dehydrogenase (ALDH). ALDH activity has been used as a marker for the quality of hematopoietic stem cell transplants. Historically, ALDH levels have been measured using live cell-permeable dyes that emit in the green region of the electromagnetic spectrum (512 nm). Thus, the reagent cannot be simultaneously utilized in cells or mice expressing green-fluorescent proteins emitting in the green fluorescent spectrum. AldeRed is a red-shifted fluorescent substrate for ALDH used for labelling viable ALDH positive cells leaving the green emission channel available for further experimentation.

Read about AldeRed in Nature

AldeRed Features and Benefits:

  • Red-shifted assay leaves green channel available for further experimentation
  • Live stem cell identification enables flow sorting of rare cell populations
  • Rapid enzymatic assay protocol

AldeRed 588-A is a fluorescent and non-toxic ALDH substratethat diffuses freely into live intact and viable cells, but remains trapped inside the cells once converted by ALDH into the corresponding acid.