| Recommended applications |
For researchers who require an animal component free system.
For researchers who will customize with their own attachment factors and/or ECM proteins/peptides
For researchers who require a minimal number of cell attachment sites
|
For researchers who require a large number of generalized cell attachment sites for their stem cell culture(s)
|
For researchers planning to incorporate and gradually release growth factors into the stem cell environment
|
| Customization |
- Amount and type of ECM proteins
- Amount and type of cell attachment factors
- Amount and type of growth factors
- Hydrogel rigidity
- Choice of cell encapsulation or top plating
|
- Amount and type of ECM proteins
- Amount and type of growth factors
- Hydrogel rigidity
- Choice of cell encapsulation or top plating
|
- Amount and type of ECM proteins
- Amount and type of growth factors
- Hydrogel rigidity
- Choice of cell encapsulation or top plating
|
| Ordering Information |
HYS020 - HyStem™ Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution)
HYS010 - HyStem™ Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution) |
HYSC020 - HyStem™-C Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution)
HYSC010 - HyStem™-C Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution) |
HYSHP020 - HyStem™-HP Cell Culture Scaffold Kit (for 7.5 mL of hydrogel scaffold solution)
HYSHP010 - HyStem™-HP Cell Culture Scaffold Trial Kit (for 2.5 mL of hydrogel scaffold solution) |
| Animal Component Free |
Yes |
No |
No |
| Cell Types tested |
- Human embryonic stem cells (H9s)
- Umbilical cord blood CD23+ stem cells
- Hepatic stem cells
- Hepatic progenitor cells
|
- Human embryonic stem cells
- Human mesenchymal stem cells
- Neural progenitor cells
- Hepatic progenitor cells
|
|
| Appropriate conditions |
Recommended for in vivo and in vitro experimentation |
Recommended for in vitro experimentation only |
Recommended for in vivo and in vitro experimentation |
| Cell Encapsulation |
Accomodates cell encapsulation (true 3D) as well as plating on top of the hydrogel for pseudo-3D growth |
| Cell Attachment |
Offers a minimal number of cell attachment sites; if desired, researchers may incorporate attachment factors of their choice. |
Enables attachment of a wide variety of cell types through Gelin-S (thiolated gelatin) |
Enables attachment of a wide variety of cell types through Gelin-S (thiolated gelatin) |
| Growth Factor Incorporation |
Researchers will customize with their own growth factors; growth factors will likely require replenishment |
Researchers will customize with their own growth factors; growth factors will likely require replenishment |
Enables ionic binding and slow release of growth factors through thiolated heparin; frequent replenishment of growth factors is not required. |
| Gelation Time |
<20 minutes |
20 minutes |
23 minutes |
| Commercially available GMP counterpart |
Yes, available through Glycosan Biosciences; www.glycosan.com |
