The Induced Pluripotent Stem Cell Design Studio (iPS CDS) group at Sigma-Aldrich leverages CompoZr® Zinc Finger Nuclease Technology, to engineer human iPS cell lines for customers to use in areas such as basic research, cell-based assays, target validation, ADME/Toxicology and drug discovery. Our mission is to facilitate customers’ research and manufacturing needs through application of iPS cell gene manipulation know how.
Zinc Finger Nucleases (ZFNs) are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination and Non-Homologous End Joining (NHEJ). By implementing established, field-proven methods, these processes can be harnessed to generate precisely targeted genomic edits, resulting in cell lines with precise and heritable gene deletions, integrations or modifications.
To engage our iPS CDS team to discuss your specific custom cell engineering needs, please fill out the following form (166 Kb PDF) and return to SCRi@sial.com.
To facilitate our clients’ research and manufacturing endeavors through expert application and support of Sigma-Aldrich CompoZr Zinc Finger Nuclease Technology (Figure 1).
Figure 1. Modifications possible using CompoZr Zinc Finger Nuclease Technology.
Why use our iPS Cell Design Studio (iPS CDS)?
Applications of Cell Lines from CDS
Our Project Workflow
|Project Phases for Standard Project||Cell Design Studio - The Sigma Effect!|
iPS CDS projects are divided into multiple phases.