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Life Science > Stem Cell Biology > Embryonic and Induced Pluripotent Stem Cells > Stemgent® Reprogramming Lentiviruses > Reprogramming Lentiviruses for Human Cells
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Stemgent Reprogramming Lentiviruses
Reprogramming Lentiviruses for Human Cells | ||
Stemgent Dox Lentivirus Set: hOKSM, concentrated (Product code ST000036)
Each doxycycline-inducible lentivirus in this set is capable of expressing one of four human transcription factors (Oct4, Klf4, Sox2 or c-Myc) under the control of the doxycycline (DOX)- inducible tetO operator when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped virus stock capable of infecting both dividing and non-dividing cells. The expression of these four transcription factors, along with the reverse tetracycline transcriptional activator (rtTA), has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell1. The DOX Inducible GFP-Lentivirus is included as a transduction control. The Stemgent Dox Lentivirus Set: hOKSM, concentrated was developed in the lab of Rudolf Jaenisch, M.D., at the Whitehead Institute at MIT1. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and techniques.
Stemgent Dox Lentivirus Set: hOKSM, concentrated (Product code ST000036)
Each doxycycline-inducible lentivirus in this set is capable of expressing one of four human transcription factors (Oct4, Klf4, Sox2 or c-Myc) under the control of the doxycycline (DOX)- inducible tetO operator when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped virus stock capable of infecting both dividing and non-dividing cells. The expression of these four transcription factors, along with the reverse tetracycline transcriptional activator (rtTA), has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell1. The DOX Inducible GFP-Lentivirus is included as a transduction control. The Stemgent Dox Lentivirus Set: hOKSM, concentrated was developed in the lab of Rudolf Jaenisch, M.D., at the Whitehead Institute at MIT1. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and techniques.
Stemgent Dox Lentivirus Set: hOKSM (Product code ST000037)
Each doxycycline-inducible lentivirus in this set is capable of expressing one of four human transcription factors (Oct4, Klf4, Sox2 or c-Myc) under the control of the doxycycline (DOX)- inducible tetO operator when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped and concentrated virus stock capable of infecting both dividing and non-dividing cells. The expression of these four transcription factors, along with the reverse tetracycline transcriptional activator (rtTA), has been shown to reprogram human adult fibroblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell1. The GFP-Lentivirus is included as a transduction control. The Stemgent Dox Lentivirus Set: hOKSM was developed in the lab of Rudolf Jaenisch, M.D., at the Whitehead Institute at MIT1. Dr. Jaenisch is a recognized leader in the study of epigenetic regulation of gene expression with numerous publications focused on ES and iPS cellular mechanisms and techniques.
Stemgent Lentivirus Set: hOKSM (Product Code ST000044)
The expression of the four human transcription factors (TFs) (Oct4, Klf4, Sox2, and c-Myc) has been shown to reprogram human adult fi broblasts to an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell.2 The Stemgent Lentivirus Set: hOKSM consists of four lentiviruses, each of which is capable of expressing one of these four factors when transduced into mammalian cells. Each unique virus is provided as a VSV-G pseudotyped virus stock capable of effi ciently transducing a wide range cell types.
Stemgent Lentivirus Set: hOSLN (Product Code ST000005)
The Stemgent Lentivirus Set: hOSLN provides the human transcription factors Oct4, Sox2, Lin28 and Nanog for use in reprogramming somatic cells into an embryonic stem (ES) cell-like state known as the induced pluripotent stem (iPS) cell3. Each virus is provided as a VSV-G pseudotyped pantrophic virus stock capable of infecting both dividing and non-dividing cells. The expression of the four transcription factors (Oct3/4, Sox2, Lin28, and Nanog) has been shown to direct a variety of differentiated cell types to become reprogrammed iPS cells3.
References
- Hockemeyer, D., Soldner, F., Cook, E.G., Gao, Q., Mitalipova, M., and Jaenisch, R. (2008) A drug-inducible system for direct reprogramming of human somatic cells to pluripotency. Cell Stem Cell 3, 346-353.
- Takahashi, K., Tanabe, K., Ohnuki, M., Narita, M., Ichisaka, T., Tomoda, K., and Yamanaka, S. (2007) Induction of pluripotent stem cells from adult human fibroblasts by defined factors. Cell 131, 861-872.
- Yu et al., Science 318:1917-1920 (2007).