Stemline^® Pluripotent Culture Medium

Defined, consistent, and feeder-independent, Stemline Pluripotent Culture Medium offers all of the performance you've come to expect in a pluripotent culture medium at a substantial savings to leading alternatives.

• Defined & Consistent Confidently culture pluripotent stem cells in a defined, controlled, consistent cellular environment, free of feeder layers.
• Significantly Reduced bFGF Stemline Pluripotent contains 80% less than the market leading alternative.
• Maintainence of Pluripotency Cells grown in Stemline Pluripotent maintain the pluripotent phenotype and express the markers of pluripotency; by minimizing spontaneous differentiation, a pure population of PSCs is maintained.
• Robust Expansion Cells grown in Stemline Pluripotent expand at rates consistent with those of cells grown in leading alternatives; these optimal rates enable timely proliferation without worry of karyotypic disruption.
• Substantial Savings Offered at a substantial savings to marketing leading altenatives, Stemline Pluripotent helps researchers conserve valuable resources and focus them on their most relevant needs.

To inquire about volume discounts, email the Sigma Stemline team or contact your local Sigma representative today.

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Substantial Savings

With all of the performance advantages of market leading alternatives, Stemline Pluripotent still enables significant savings. Stemline Pluripotent is priced 20% lower than the top alternative pluripotent medium, leading to substantial savings for those who make Stemline their medium of choice. Additional savings can be earned by committing to larger purchase volumes.

Maintain Normal Karyotype

Human iPS cells were cultured with Stemline Pluripotent Stem Cell Culture Medium for 7 passages. Analysis by GTL banding indicate normal human female karyotype.

Maintain Differentiation Capability

Cells grown in Stemline Pluripotent Stem Cell Culture Medium successfully differentiate to cells of the three germ layers. Human iPS cells (Product No. IPSC0028) were cultured in Stemline Pluripotent Culture Medium for 10 passages prior to differentiation. Cells underwent embryoid body differentiation in the presence of FBS (Product No. F2442). RNA was collected at Day 21 of EB differentiation and qRT-PCR data was normalized to cyclophilin A. Fold increase values were compared to passage 10 undifferentiated pluripotent stem cells.