Life Science Reagents for Protein Expression and Purification

Find all the products you need to carry out successful Protein Expression and Purification experiments in this one convenient location. All products are quality and availability assured. Whether you are performing isolation, purification, or expression the products to complete your experiment are below. This bundle of Protein Expression and Purification products includes:
  • Acids & Bases
  • Acrylamides
  • Buffers
  • Carbohydrates
  • Cell Lysis
  • Detergents
  • General Reagents
  • Protease Inhibitors
  • Solvents

Acids & Bases

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F0507 Formic acid reagent grade, ≥95%
A9967 Glacial acetic acid meets USP testing specifications
H1758 Hydrochloric acid 36.5-38.0%, BioReagent, for molecular biology
438081 Phosphoric acid ACS reagent, ≥85 wt. % in H2O Green Alternative
P5958 Potassium hydroxide BioXtra, ≥85% KOH basis
S8045 Sodium hydroxide BioXtra, ≥98% (acidimetric), pellets (anhydrous)
T9159 Trichloroacetic acid BioXtra, ≥99.0%

Acrylamides

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A3553 Acrylamide for electrophoresis, ≥99% (HPLC), powder
A9099 Acrylamide for molecular biology, ≥99% (HPLC)
M7279 N,N′-Methylenebisacrylamide powder, for molecular biology, for electrophoresis, ≥99.5%

Buffers / Buffer Salts

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A1542 Ammonium acetate for molecular biology, ≥98%
A2706 Ammonium acetate solution for molecular biology, 7.5 M
A6141 Ammonium bicarbonate ReagentPlus®, ≥99.0%
B6768 Boric acid BioReagent, for molecular biology, suitable for cell culture, suitable for plant cell culture, ≥99.5%
C3306 Calcium chloride dihydrate for molecular biology, ≥99%
G3272 Guanidine hydrochloride for molecular biology, ≥99%
H4034 HEPES BioPerformance Certified, ≥99.5% (titration), cell culture tested
H3784 HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.5%
H3537 HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
I5513 Imidazole for molecular biology, ≥99% (titration)
M5661 Magnesium acetate tetrahydrate for molecular biology, ≥99%
M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M
M3409 Magnesium sulfate solution for molecular biology, 1.00 M±0.04 M
M3183 MOPS BioPerformance Certified, cell culture tested, ≥99.5% (titration)
M9024 MOPS sodium salt BioPerformance Certified, suitable for cell culture, ≥99.5%
M1442 MOPS solution BioPerformance Certified, 1 M, suitable for cell culture
P5493 Phosphate buffered saline 10× concentrate, BioPerformance Certified, suitable for cell culture
P3813 Phosphate buffered saline powder, pH 7.4, for preparing 1 L solutions
P1190 Potassium acetate for molecular biology, ≥99.0%
P9541 Potassium chloride for molecular biology, ≥99.0%
P9791 Potassium phosphate monobasic for molecular biology, ≥98.0%
S3272 Sodium acetate for electrophoresis, ≥99%, crystalline
S3014 Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)
S3264 Sodium phosphate dibasic for molecular biology, ≥98.5% (titration)
T9534 Triethanolamine hydrochloride BioXtra, ≥99.5% (titration)
T6664 Tris Buffered Saline pH 8.0, powder
T6066 Trizma® base BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)
T5941 Trizma® hydrochloride BioPerformance Certified, cell culture tested, ≥99.0% (titration)

Cell Lysis

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BCA1 Bicinchoninic Acid Kit for Protein Determination for 200-1000 μg/ml protein
C2978 CelLytic M Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization.
B7435 CelLytic B Cell Lysis Reagent For bacterial cell lysis, standard strength
B7310 CelLytic B Cell Lysis Reagent For bacterial cell lysis, 2× concentrate
C8740 CelLytic B Cell Lysis Reagent For bacterial cell lysis, 10× concentrate
C4482 CelLytic Y Cell Lysis Reagent For yeast cells
C1990 CelLytic Express
C5236 CelLytic IB Inclusion Body Solubilization Reagent
CE0050 CelLytic MEM Protein Extraction Kit
C3228 CelLytic MT Cell Lysis Reagent For mammalian tissues
CB0050 CelLytic B Plus Kit For bacterial lysis
C7988 Monoclonal Anti-Cre antibody produced in mouse ~2 mg/mL, clone 7-23, purified immunoglobulin, buffered aqueous solution
C3956 Anti-c-Myc antibody produced in rabbit ~0.5 mg/mL, affinity isolated antibody, buffered aqueous solution
R0278 RIPA Buffer

Detergents

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P1254 Brij® L23 suitable for Stein-Moore chromatography
L3771 Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
T8787 Triton X-100 for molecular biology
P9416 TWEEN® 20 for molecular biology, viscous liquid

General Reagents

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A8199 N-Acetyl-L-cysteine BioXtra, ≥99% (TLC)
A7699 Adenosine 5′-triphosphate disodium salt hydrate BioXtra, ≥99% (HPLC), from microbial
A7906 Bovine Serum Albumin heat shock fraction, pH 7, ≥98%
A3156 Bovine Serum Albumin lyophilized powder, γ-irradiated, Globulin Free, BioXtra, suitable for cell culture
A3428 Aprotinin from bovine lung lyophilized powder, 3-8 TIU/mg solid, BioReagent, suitable for cell culture
B5525 Bromophenol Blue sodium salt
C3306 Calcium chloride dihydrate for molecular biology, ≥99%
D5905 3,3′-Diaminobenzidine tetrahydrochloride tablet, 10 mg substrate per tablet
D2438 Dimethyl sulfoxide sterile-filtered, BioPerformance Certified, meets EP, USP testing specifications, suitable for hybridoma
D9779 DL-Dithiothreitol for molecular biology, ≥98% (HPLC), ≥99% (titration)
AMPD1 DNase I Amplification Grade
E5134 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration)
E7889 Ethylenediaminetetraacetic acid disodium salt solution for molecular biology, 0.5 M in H2O, DNase, RNase, NICKase and protease, none detected
E3889 Ethylene glycol-bis(2-aminoethylether)-N,N,N′,N′-tetraacetic acid for molecular biology, ≥97.0%
F2442 Fetal Bovine Serum USA origin, sterile-filtered, suitable for cell culture, suitable for hybridoma
G7528 D-(+)-Glucose BioXtra, ≥99.5% (GC)
G5516 Glycerol for molecular biology, ≥99%
G7403 Glycine BioXtra, ≥99% (titration)
I1149 Iodoacetamide BioUltra
L8912 L-Leucine from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%
L9783 Leupeptin hydrochloride microbial, ≥90% (HPLC)
M5661 Magnesium acetate tetrahydrate for molecular biology, ≥99%
M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M
M3409 Magnesium sulfate solution for molecular biology, 1.00 M±0.04 M
M3942 MegaCell Dulbecco’s Modified Eagle’s Medium liquid, sterile-filtered, suitable for cell culture
M3148 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
P7626 Phenylmethanesulfonyl fluoride ≥98.5% (GC)
P7170 Ponceau S solution BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid
P1190 Potassium acetate for molecular biology, ≥99.0%
P9541 Potassium chloride for molecular biology, ≥99.0%
P8131 Potassium hexacyanoferrate(III) ReagentPlus®, ~99%
P8038 Proteinase, bacterial Type XXIV, 7.0-14.0 units/mg solid, lyophilized powder
S3401 Sample Buffer, Laemmli 2× Concentrate
S7795 Sodium carbonate BioXtra, ≥99.0%
S7920 Sodium fluoride BioXtra, ≥99%
S8170 Sodium nitrate BioXtra, ≥99.0%
S6422 Sodium pyrophosphate tetrabasic decahydrate BioXtra, 99.0-103.0%
S6672 Sodium thiosulfate pentahydrate BioXtra, ≥99.5%
S0389 Sucrose for molecular biology, ≥99.5% (GC)
T0440 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for ELISA peroxidase substrate
T4648 Thrombin from bovine plasma lyophilized powder, 40-300 NIH units/mg protein (biuret)
T9534 Triethanolamine hydrochloride BioXtra, ≥99.5% (titration)
T8658 Trypsin from bovine pancreas suitable for protein sequencing, lyophilized powder
U5378 Urea powder, BioReagent, for molecular biology, suitable for cell culture
W4502 Water Molecular Biology Reagent

Labware

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Z722073 Pall Nanosep® centrifugal device with Omega membrane MWCO 10 kDa
Z340006 Sigma 2-D electrophoresis set Complete Set
SC1000 SigmaPrep spin column storage temp.: 20-25°C, pkg of 1 kit
MC1000 SigmaPrep spin column kit with break-away tip storage temp.: 20-25°C, pkg of 1 kit
Z375063 Vertical electrophoresis unit, adjustable height, single gel gel W × H 16.5 cm × 14.5-28 cm, CE-approved

Protease Inhibitors

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C7268 Chymostatin microbial
E3132 E-64 protease inhibitor
L9783 Leupeptin hydrochloride microbial, ≥90% (HPLC)
MSSAFE MS-SAFE Protease and Phosphatase Inhibitor
P8340 Protease Inhibitor Cocktail for use with mammalian cell and tissue extracts, DMSO solution
P8849 Protease Inhibitor Cocktail for use in purification of Histidine-tagged proteins, DMSO solution
P9599 Protease Inhibitor Cocktail for plant cell and tissue extracts, DMSO solution
P8215 Protease Inhibitor Cocktail for use with fungal and yeast extracts, DMSO solution
P1860 Protease Inhibitor Cocktail for use in tissue culture media, DMSO solution
I3911 Protease Inhibitor Cocktail, Animal Component Free for general use, lyophilized powder
I3786 Protease Inhibitor Cocktail, Animal Component Free for use with mammalian cell and tissue extract, DMSO solution
P2714 Protease Inhibitor Cocktail powder for general use, lyophilized powder
P8465 Protease Inhibitor Cocktail powder for use with bacterial cell extracts, lyophilized powder
S8830 SIGMAFAST Protease Inhibitor Cocktail Tablets, EDTA-Free for use in purification of Histidine-tagged proteins
S8820 SIGMAFAST Protease Inhibitor Tablets For General Use

Solvents

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496189 Chloroform biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
D4551 N,N-Dimethylformamide for molecular biology, ≥99%
E7023 Ethyl alcohol, Pure 200 proof, for molecular biology
494437 Methanol suitable for protein sequencing, BioReagent, ≥99.93%
P1037 Phenol for molecular biology
I9516 2-Propanol for molecular biology, BioReagent, ≥99.5%