Life Science Reagents for Cloning

Find all the products you need to carry out successful Cloning experiments in this one convenient location. All products are quality and availability assured. This bundle of Cloning products includes:

  • Acids & Bases
  • Agarose
  • Buffers
  • Competent Cells
  • Enzymes
  • General Reagents

For Cloning Systems, Expression Vectors and more, please visit our Cloning Expression Node.

Acids & Bases

Product #

Description

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H1758 Hydrochloric acid 36.5-38.0%, BioReagent, for molecular biology
    • P5811 Phosphoric acid BioReagent, suitable for insect cell culture, 85%
    • P5958 Potassium hydroxide BioXtra, ≥85% KOH basis
    • S2770 Sodium hydroxide solution 1.0 N, BioReagent, suitable for cell culture

    • Agarose

    Product #

    Description

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    A9539 Agarose For routine use
    • A6013 Agarose Type I, low EEO
    • A6877 Agarose Type II, Medium EEO
    • A0169 Agarose Type I-A, low EEO
    • A4718 Agarose High resolution, DNase, RNase, NICKase, none detected
    • A7431 Agarose Wide range/Standard 3:1, DNase, RNase, NICKase, none detected
    • A5093 Agarose low EEO, for routine use

    • Antibiotics

    Product #

    Description

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    A0166 Ampicillin sodium salt powder, BioReagent, suitable for cell culture
    • C1863 Chloramphenicol Biotechnology Performance Certified, suitable for plant cell culture
    • D9891 Doxycycline hyclate ≥98% (TLC)
    • G5013 G 418 disulfate salt potency: ≥450 μg per mg (dried basis)
    • A1720 G 418 disulfate salt powder, BioReagent, suitable for cell culture
    • H7772 Hygromycin B from Streptomyces hygroscopicus lyophilized powder
    • H0654 Hygromycin B solution from Streptomyces hygroscopicus ≥60% (HPAE), 45-60 mg/mL in H2O
    • K1377 Kanamycin sulfate from Streptomyces kanamyceticus powder, BioReagent, suitable for cell culture, suitable for plant cell culture
    • P9620 Puromycin dihydrochloride Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, 0.2 μm filtered
    • P8833 Puromycin dihydrochloride from Streptomyces alboniger powder, BioReagent, suitable for cell culture
    • S9137 Streptomycin sulfate salt powder, BioReagent, suitable for cell culture
    • T7660 Tetracycline hydrochloride powder, BioReagent, suitable for cell culture

    • Broth / Media

    Product #

    Description

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    A7002 Agar ash 2.0-4.5%
    • A5306 Bacteriological agar for molecular biology
    • C6905 β-Casein from bovine milk BioUltra, ≥98% (PAGE)
    • T2559 EZMix™ Tryptone Pancreatic digest of casein
    • S7313 S-Gal® sodium salt powder
    • L2897 LB Agar powder
    • L5667 LB Agar Ampicillin-100, Plates pre-poured agar plates with 100 ug/ml ampicillin
    • L7533 LB Agar EZMix™ Powder
    • L0543 LB Agar Kanamycin-50, Plates pre-poured agar plates with 50 ug/ml kanamycin
    • L5542 LB Agar Plates
    • L8795 LB Agar Tetracycline-10, Plates pre-poured agar plates with 10 μg/ml tetracycline
    • L7275 LB Broth tablet
    • L3022 LB Broth powder
    • L2542 LB Broth Liquid Medium
    • L7658 LB Broth EZMix™ Powder quick-dissolve, dust-free formulation
    • L3147 Luria Agar
    • L3522 Luria Broth Miller′s Modification, 10 g/L NaCl
    • C0626 N-Z-Amine® A from bovine milk, Casein enzymatic hydrolysate
    • N3643 NZCYM Broth powder
    • P5905 Peptone from animal tissue from meat, powder, suitable for cell culture
    • Y1626 Select Yeast Extract EZMix™ powder
    • C4478 S-Gal®/LB Agar Blend
    • S9938 S-Gal®/LB Agar Blend, without IPTG
    • S1813 S-Gal®/LB Agar/Kanamycin Blend powder
    • S1797 SOC Medium For use in transformation
    • T5574 Terrific Broth Liquid Medium
    • T9179 Terrific Broth, Modified EZMix™ Powder TB; quick dissolve, dust-free formulation
    • T7293 Tryptone Microbiologically tested.
    • T9410 Tryptone Pancreatic digest of casein
    • Y1625 Yeast Extract powder
    • Y1003 2xYT Medium
    • Y2627 2X YT Microbial Medium EZMix™ Powder nutrient rich, quick dissolve, dust free formulation

    • Buffer / Buffer Salts

    Product #

    Description

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    A1542 Ammonium acetate for molecular biology, ≥98%
    • B4679 BIS-TRIS propane BioReagent, cell culture tested, ≥99.0% (titration)
    • H4034 HEPES Biotechnology Performance Certified, ≥99.5% (titration), cell culture tested
    • H3784 HEPES sodium salt BioReagent, suitable for cell culture, ≥99.5% (titration)
    • H3537 HEPES solution 1 M, BioReagent, suitable for cell culture, suitable for molecular biology, 0.2 μm filtered
    • M2545 Magnesium acetate tetrahydrate BioXtra, ≥99%
    • M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M
    • M3409 Magnesium sulfate solution for molecular biology, 1.00 M±0.04 M
    • P1190 Potassium acetate for molecular biology, ≥99.0%
    • P9541 Potassium chloride for molecular biology, ≥99.0%
    • S2889 Sodium acetate anhydrous
    • S3014 Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)
    • C8532 Sodium citrate tribasic dihydrate for molecular biology, suitable for amino acid analysis, ≥99%
    • S3264 Sodium phosphate dibasic for molecular biology, ≥98.5% (titration)
    • T9285 Tris-EDTA buffer solution 100 ×, for molecular biology
    • T6066 Trizma® base Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)
    • Z0173 Zinc chloride BioReagent, for molecular biology, ≥97.0%

    • Competent Cells

    Product #

    Description

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    B2685 BL21 Competent Cells, Uni-pack T1R
    • G2919 GC10™ Competent Cells Uni-pack
    • G3169 GC5™ Competent Cells Uni-pack
    • J3895 JM109 Competent Cells, Uni-pack

    • Enzymes

    Product #

    Description

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    D2886 DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution
    • P2308 Proteinase K from Tritirachium album lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
    • R0260 BamH I from Bacillus amyloliquefaciens H buffered aqueous glycerol solution
    • R6265 EcoR I from Escherichia coli BS5 buffered aqueous glycerol solution
    • R1137 Hind III from Haemophilus influenzae buffered aqueous glycerol solution
    • R8506 Not I from Nocardia otidiscaviarum buffered aqueous glycerol solution
    • R7023 Pst I from Providencia stuartii buffered aqueous glycerol solution
    • R0754 Sal I from Streptomyces albus G buffered aqueous glycerol solution
    • R6379 Xho I from Xanthomonas holcicola buffered aqueous glycerol solution
    • R6513 Ribonuclease A from bovine pancreas ≥70 Kunitz units/mg protein, salt-free, lyophilized powder
    • T4427 Terminal Transferase from calf thymus buffered aqueous glycerol solution

    • General Reagents

    Product #

    Description

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    A3059 Albumin from bovine serum lyophilized powder, essentially globulin free, essentially protease free, ≥98% (agarose gel electrophoresis)
    • APF Alkaline Phosphatase Detection Kit, Fluorescence
    • B3928 Blue-White Select™ Screening Reagent
    • B4252 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%, powder
    • C3032 Cesium chloride for molecular biology, ≥99% (silver nitrate titration)
    • C2432 Chloroform contains 100-200 ppm amylenes as stabilizer, ≥99.5%
    • D0632 DL-Dithiothreitol ≥98% (TLC), ≥99.0% (titration)
    • D9779 DL-Dithiothreitol for molecular biology, ≥98% (TLC), ≥99% (titration)
    • E7023 Ethanol 200 proof (absolute), for molecular biology
    • E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O
    • E5134 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration)
    • S9811 S-Gal® powder
    • G7663 Gelatin blocking buffer for molecular biology, powder blend
    • G5400 D-(+)-Glucose ≥99% (GC)
    • G5516 Glycerol for molecular biology, ≥99%
    • I5502 Isopropyl β-D-1-thiogalactopyranoside ≥99% (TLC)
    • D9893 pBR322 Plasmid DNA from E. coli RRI lyophilized powder
    • P1037 Phenol for molecular biology
    • P2069 Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA. for molecular biology
    • P5413 Poly(ethylene glycol) for molecular biology, average mol wt 8,000
    • 82514 Protein Hydrolysate Amicase® for microbiology
    • L4390 Sodium dodecyl sulfate for molecular biology, ≥98.5% (GC)
    • S6021 D-Sorbitol for molecular biology, ≥98%
    • W4502 Water Molecular Biology Reagent
    • YEAST1 Yeast Transformation Kit

    • Labware

    Product #

    Description

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    68061 Anaerobic atmosphere generation bags for microbiology
    • 28029 Anaerobic jar for microbiology
    • C1059 Cloning cylinders, glass volume 150 μL
    • C2059 Cloning cylinders, glass volume 250 μL
    • Z358762 Culture dishes size 100 mm × 25 mm, sterile, polystyrene
    • G8772 Glass beads, acid-washed 425-600 μm (30-40 U.S. sieve)
    • G4649 Glass beads, acid-washed ≤106 μm (−140 U.S. sieve)
    • G1145 Glass beads, acid-washed 150-212 μm (70-100 U.S. sieve)
    • Z376779 Lazy-L Spreader™ 10 per pouch
    • D6789 Nunclon® Δ Multidishes 4 wells, flat bottom
    • Z359947 Pellet pestles blue polypropylene (Autoclavable)
    • Z359971 Pellet pestles Cordless motor
    • P5606 Petri dishes, polystyrene size 100 mm × 20 mm, beveled stacking rings
    • C7983 Scienceware® cloning cylinders, polystyrene I.D. × H 4.7 mm × 8 mm
    • Z374431 Scienceware® cloning discs size 3 mm ( 1/8 in.)