Life Science Reagents for Cloning

Find all the products you need to carry out successful Cloning experiments in this one convenient location. All products are quality and availability assured. This bundle of Cloning products includes:

  • Acids & Bases
  • Agarose
  • Buffers
  • Competent Cells
  • Enzymes
  • General Reagents

For Cloning Systems, Expression Vectors and more, please visit our Cloning Expression Node.

Acids & Bases

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H1758 Hydrochloric acid 36.5-38.0%, BioReagent, for molecular biology
P5811 Phosphoric acid BioReagent, suitable for insect cell culture, 85%
P5958 Potassium hydroxide BioXtra, ≥85% KOH basis
S2770 Sodium hydroxide solution 1.0 N, BioReagent, suitable for cell culture

Agarose

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A9539 Agarose BioReagent, for molecular biology, low EEO
A6013 Agarose Type I, low EEO
A6877 Agarose Medium EEO, for molecular biology
A0169 Agarose Type I-A, low EEO
A4718 Agarose High resolution, for molecular biology
A7431 Agarose BioReagent, for molecular biology, Wide range/Standard 3:1

Antibiotics

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ROAMP Ampicillin
A0166 Ampicillin sodium salt powder, BioReagent, suitable for cell culture
C1863 Chloramphenicol Biotechnology Performance Certified, suitable for plant cell culture
D9891 Doxycycline hyclate ≥98% (HPLC)
G5013 G 418 disulfate salt potency: ≥450 μg per mg (dried basis)
A1720 G 418 disulfate salt powder, BioReagent, suitable for cell culture
H7772 Hygromycin B from Streptomyces hygroscopicus lyophilized powder
H0654 Hygromycin B solution from Streptomyces hygroscopicus ≥60% (HPLC), 45-60 mg/mL in H2O
K1377 Kanamycin sulfate from Streptomyces kanamyceticus powder, BioReagent, suitable for cell culture, suitable for plant cell culture
P9620 Puromycin dihydrochloride Ready Made Solution, from Streptomyces alboniger, 10 mg/mL in H2O, 0.2 μm filtered
P8833 Puromycin dihydrochloride from Streptomyces alboniger powder, BioReagent, suitable for cell culture
S9137 Streptomycin sulfate salt powder, BioReagent, suitable for cell culture
T7660 Tetracycline hydrochloride powder, BioReagent, suitable for cell culture

Broth / Media

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Y2627 2xYT Medium EZMix Powder microbial growth medium
A7002 Agar ash 2.0-4.5%
A5306 Bacteriological agar for molecular biology
C6905 β-Casein from bovine milk BioUltra, ≥98% (PAGE)
T2559 EZMix Tryptone Pancreatic digest of casein
S7313 S-Gal® sodium salt reagent for selection of recombinant bacterial clones
L5667 LB Agar Ampicillin-100, Plates pre-poured agar plates with 100 μg/ml ampicillin
L0543 LB Agar Kanamycin-50, Plates pre-poured agar plates with 50 ug/ml kanamycin
L5542 LB Agar Plates pre-poured agar plates for E. coli
L8795 LB Agar Tetracycline-10, Plates pre-poured agar plates with 10 μg/ml tetracycline
L7275 LB Broth (Lennox) Tablet microbial growth medium
L7658 LB Broth (Lennox) EZMix powder microbial growth medium
L3022 LB Broth (Lennox) Powder microbial growth medium
L3522 LB Broth (Miller) Powder microbial growth medium
L2542 LB Broth (Miller) Liquid microbial growth medium
L2897 LB Broth with agar (Lennox) Powder microbial growth medium
L7533 LB Broth with agar (Lennox) EZMix powder microbial growth medium
L3147 LB Broth with agar (Miller) Powder microbial growth medium
C0626 N-Z-Amine® A from bovine milk, Casein enzymatic hydrolysate
N3643 NZCYM Broth Powder microbial growth medium
P5905 Peptone from animal tissue from meat, BioReagent, suitable for cell culture, suitable for plant cell culture
Y1626 Select Yeast Extract, EZMix powder for use in microbial growth medium
C4478 S-Gal®/LB Agar Blend reagent for selection of recombinant bacterial clones
S9938 S-Gal®/LB Agar Blend, without IPTG
S1813 S-Gal®/LB Agar/Kanamycin Blend powder
S1797 SOC Medium For use in transformation
T9179 Terrific Broth EZMix powder microbial growth medium
T5574 Terrific Broth Liquid microbial growth medium
T7293 Tryptone Microbiologically tested.
T9410 Tryptone Pancreatic digest of casein
Y1625 Yeast Extract for use in microbial growth medium
Y1003 2x YT medium Liquid microbial growth medium

Buffer / Buffer Salts

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A1542 Ammonium acetate for molecular biology, ≥98%
B4679 BIS-TRIS propane BioPerformance Certified, cell culture tested, ≥99.0%
H4034 HEPES BioPerformance Certified, ≥99.5% (titration), cell culture tested
H3784 HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.5%
H3537 HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered
M2545 Magnesium acetate tetrahydrate BioXtra, ≥99%
M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M
M3409 Magnesium sulfate solution for molecular biology, 1.00 M±0.04 M
P1190 Potassium acetate for molecular biology, ≥99.0%
P9541 Potassium chloride for molecular biology, ≥99.0%
S2889 Sodium acetate anhydrous, for molecular biology, ≥99%
S3014 Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)
C8532 Sodium citrate tribasic dihydrate for molecular biology, ≥99%
S3264 Sodium phosphate dibasic for molecular biology, ≥98.5% (titration)
T9285 Tris-EDTA buffer solution 100X concentrate for Northern and Southern blotting
T6066 Trizma® base BioPerformance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)
Z0173 Zinc chloride BioReagent, for molecular biology, ≥97.0%

Competent Cells

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G2919 GC10 Competent Cells for generation of cDNA libraries and DNA plasmid production
G3169 GC5 Competent Cells for generation of cDNA libraries and DNA plasmid production
J3895 JM109 Competent Cells for routine DNA plasmid production
B2685 transformation for protein expression and DNA plasmid production

General Reagents

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A3059 Bovine Serum Albumin heat shock fraction, protease free, essentially globulin free, pH 7, ≥98%
APF Alkaline Phosphatase Detection Kit, Fluorescence
B3928 Blue-White Select Screening Reagent for selection of recombinant bacterial clones
B4252 5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside ≥98%, powder
C3032 Cesium chloride for molecular biology, ≥99% (silver nitrate titration)
C2432 Chloroform contains 100-200 ppm amylenes as stabilizer, ≥99.5%
D0632 DL-Dithiothreitol ≥98% (HPLC), ≥99.0% (titration)
D9779 DL-Dithiothreitol for molecular biology, ≥98% (HPLC), ≥99% (titration)
E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O
E7023 Ethyl alcohol, Pure 200 proof, for molecular biology
E5134 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration)
S9811 S-Gal® reagent for selection of recombinant bacterial clones
G7663 Gelatin blocking buffer for Western blotting, powder blend
G5400 D-(+)-Glucose ≥99% (GC)
G5516 Glycerol for molecular biology, ≥99%
I5502 Isopropyl β-D-1-thiogalactopyranoside ≥99% (TLC)
D9893 pBR322 Plasmid DNA from E. coli RRI lyophilized powder
P1037 Phenol for molecular biology
P2069 Phenol:Chloroform:Isoamyl Alcohol 25:24:1 Saturated with 10 mM Tris, pH 8.0, 1 mM EDTA Supplied with Equilibration buffer, for molecular biology
P5413 Poly(ethylene glycol) for molecular biology, average mol wt 8,000
82514 Protein Hydrolysate Amicase® for microbiology
S6021 D-Sorbitol for molecular biology, ≥98%
W4502 Water Molecular Biology Reagent
YEAST1 Yeast Transformation Kit reagents for introducing plasmid DNA into yeast

Labware

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68061 Anaerobic atmosphere generation bags for microbiology
28029 Anaerobic jar for microbiology
C1059 Cloning cylinders, glass volume 150 μL
C2059 Cloning cylinders, glass volume 250 μL
Z358762 Culture dishes size 100 mm × 25 mm, sterile, polystyrene
G8772 Glass beads, acid-washed 425-600 μm (30-40 U.S. sieve)
G4649 Glass beads, acid-washed ≤106 μm (−140 U.S. sieve)
G1145 Glass beads, acid-washed 150-212 μm (70-100 U.S. sieve)
Z376779 Lazy-L Spreader 10 per pouch
D6789 Nunclon® Δ Multidishes 4 wells, flat bottom
Z359947 Pellet pestles blue polypropylene (Autoclavable)
Z359971 Pellet pestles Cordless motor
P5606 Petri dishes, polystyrene size 100 mm × 20 mm, slippable
C7983 Scienceware® cloning cylinders, polystyrene I.D. × H 4.7 mm × 8 mm
Z374431 Scienceware® cloning discs diam. 3.2 mm ( 1/8 in.)

Life Science Reagents for Cloning Enzymes

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D2886 DNA Ligase from T4-infected Escherichia coli buffered aqueous glycerol solution
P2308 Proteinase K from Tritirachium album lyophilized powder, BioUltra, ≥30 units/mg protein, for molecular biology
R0260 BamH I from Bacillus amyloliquefaciens H Restriction Enzyme
R6265 EcoR I from Escherichia coli BS5 Restriction Enzyme
R1137 Hind III from Haemophilus influenzae Restriction Enzyme
R8506 Not I from Nocardia otidiscaviarum Restriction Enzyme
R7023 Pst I from Providencia stuartii Restriction Enzyme
R0754 Sal I from Streptomyces albus G Restriction Enzyme
R6379 Xho I from Xanthomonas holcicola Restriction Enzyme
R6513 Ribonuclease A from bovine pancreas for molecular biology, ≥70 Kunitz units/mg protein, lyophilized
T4427 Terminal Transferase from calf thymus buffered aqueous glycerol solution