Life Science Reagents for Northern and Southern Blotting

Find all the products you need to carry out successful Blotting experiments in this one convenient location. All products are quality and availability assured. This bundle of Northern and Southern blotting products includes:

  • Acids & Bases
  • Agaroses
  • Buffers
  • Memebranes
  • Stains & Dyes
  • Labware
  • General Reagents

Acids & Bases

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H1758 Hydrochloric acid 36.5-38.0%, BioReagent, for molecular biology
S8045 Sodium hydroxide BioXtra, ≥98% (acidimetric), pellets (anhydrous)

Agarose

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A9539 Agarose For routine use
A6013 Agarose Type I, low EEO
A6877 Agarose Type II, Medium EEO
A0169 Agarose Type I-A, low EEO
A4718 Agarose High resolution, DNase, RNase, NICKase, none detected
A7431 Agarose Wide range/Standard 3:1, DNase, RNase, NICKase, none detected
A2576 Agarose Type IX-A, Ultra-low Gelling Temperature
A5304 Agarose for electrophoresis
A5093 Agarose low EEO, for routine use
A0701 Agarose, low gelling temperature Type VII-A

Buffer / Buffer Salts

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B6429 10× Blocking Buffer 10 ×
N1531 Denaturation Solution for Neutral Southern Transfer
H4034 HEPES Biotechnology Performance Certified, ≥99.5% (titration), cell culture tested
H3784 HEPES sodium salt BioReagent, suitable for cell culture, ≥99.5% (titration)
H3662 HEPES sodium salt solution 1M, BioReagent, suitable for cell culture
H3537 HEPES solution 1 M, BioReagent, suitable for cell culture, suitable for molecular biology, 0.2 μm filtered
H7140 Hybridization Solution, 2× Concentrate, Buffered with SSC 200μg per mL DNA in 10× SSC, 2× Denhardt′s
M1028 Magnesium chloride solution for molecular biology, 1.00 M±0.01 M
M3183 MOPS Biotechnology Performance Certified, cell culture tested, ≥99.5% (titration)
M9024 MOPS sodium salt BioReagent, suitable for cell culture, ≥99.5% (titration)
M1442 MOPS solution BioReagent, 1 M, for molecular biology, suitable for cell culture
N6019 Neutralizing buffer for Southern blots powder blend
H7033 PerfectHyb Plus Hybridization Buffer liquid, 1 ×
P5368 Phosphate buffered saline BioReagent, pH 7.4, for molecular biology
P5493 Phosphate buffered saline 10× concentrate, BioReagent, suitable for cell culture, for molecular biology
P3813 Phosphate buffered saline powder, pH 7.4
P9541 Potassium chloride for molecular biology, ≥99.0%
P9791 Potassium phosphate monobasic for molecular biology, ≥98.0%
P1415 Prehybridization Solution, 2× Concentrate, Buffered with SSC
S2889 Sodium acetate anhydrous
S3014 Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)
S6191 Sodium chloride Biotechnology Performance Certified, ≥99.5% (titration), Cell Culture Tested
C8532 Sodium citrate tribasic dihydrate for molecular biology, suitable for amino acid analysis, ≥99%
S3264 Sodium phosphate dibasic for molecular biology, ≥98.5% (titration)
S6566 Sodium phosphate monobasic Biotechnology Performance Certified, Cell Culture Tested
S7778 Sodium phosphate tribasic dodecahydrate BioXtra, ≥98.0% (titration)
S6639 SSC Buffer 20× Concentrate Saline-Sodium Citrate buffer, made with ultrapure water
S2015 SSPE Buffer 20× Concentrate
T9650 Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, non-sterile; 0.2 μm filtered
T4415 Tris-Borate-EDTA buffer BioReagent, suitable for electrophoresis, 10× concentrate
T5941 Trizma® hydrochloride for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)
T6066 Trizma® base Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

General Reagents

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B4287 Albumin from bovine serum BioReagent, for molecular biology
B2518 Albumin, Acetylated from bovine serum lyophilized powder, suitable for (for molecular biology)
A0410 Aminoallyl-dUTP sodium salt ≥85%, lyophilized powder
A5660 Aminoallyl-UTP sodium salt ≥75%, lyophilized powder
C0712 CDP-Star® Chemiluminescent Substrate 0.25 mM in H2O
C3032 Cesium chloride for molecular biology, ≥99% (silver nitrate titration)
496189 Chloroform biotech. grade, ≥99.8%, contains 0.5-1.0% ethanol as stabilizer
D2532 Denhardt’s Solution 50× Concentrate for molecular biology, liquid, A 1% solution of BSA, Ficoll® and PVP
D9905 Denhardt’s Solution Lyophilized Powder for molecular biology
D3287 Deoxyribonucleic acid, single stranded from human placenta For hybridization
D8906 Dextran sulfate sodium salt from Leuconostoc spp. for molecular biology, average Mw >500,000 (dextran starting material), contains 0.5-2% phosphate buffer
494429 Dimethyl sulfoxide biotech. grade, 99.8%
E7023 Ethanol 200 proof (absolute), for molecular biology
E5134 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration)
F8775 Formaldehyde solution for molecular biology, 36.5-38% in H2O
G5516 Glycerol for molecular biology, ≥99%
M3148 2-Mercaptoethanol for molecular biology, for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration)
M7154 2-Mercaptoethanol for electrophoresis
P1037 Phenol for molecular biology
P5413 Poly(ethylene glycol) for molecular biology, average mol wt 8,000
P5288 Polyvinylpyrrolidone for molecular biology, nucleic acid hybridization tested, mol wt 360,000
L4390 Sodium dodecyl sulfate for molecular biology, ≥98.5% (GC)
T3411 Tetramethylammonium chloride solution for molecular biology
T9281 N,N,N′,N′-Tetramethylethylenediamine BioReagent, suitable for electrophoresis, ~99%
W4502 Water Molecular Biology Reagent
W3513 Water Biotechnology Performance Certified

Labware

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Z340502 Semi-dry blotter Blotter unit
B2529 Semi-dry blotter Complete unit
B2157 Wet Blotters Mini-blotter

Memebranes / Blotting Paper

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N1281 BioBond Nylon transfer membrane roll size 20 cm × 3.5 m
N7892 Nitrocellulose membranes for blotting pore size 0.2 μm, size 15 cm × 15 cm
N8017 Nitrocellulose membranes for blotting pore size 0.2 μm, size 20 cm × 20 cm
N8267 Nitrocellulose membranes for blotting pore size 0.45 μm, size 15 cm × 15 cm
Z613916 Whatman® gel blotting papers, Grade GB005 GB005 blotting sheets, 58 x 58 cm, 25/pk
Z613738 Whatman® Optitran® reinforced nitrocellulose membranes Rolls - BA-S 83, pore size 0.2 μm, W × L 30 cm × 3 m
Z670979 Whatman® Protran® nitrocellulose membranes Rolls - BA83, pore size 0.2 μm, W × L 20 cm × 3 m
Z613657 Whatman® Protran® nitrocellulose membranes Rolls - BA83, pore size 0.2 μm, W × L 30 cm × 3 m
Z613630 Whatman® Protran® nitrocellulose membranes Rolls - BA85, pore size 0.45 μm, W × L 30 cm × 3 m
Z671088 Whatman® Westran® PVDF membranes Westran Clear Signal, 0.45 μm PVDF blotting membrane, 30 cm x 3 m roll, 1/pk

Stains & Dyes

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E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O
01494 Nancy-520 ≥97.0% (HPCE)
S9430 SYBR® Green I nucleic acid gel stain 10,000 × in DMSO