Life Science Reagents for Western Blotting

Find all the products you need to carry out successful Western Blot assays in this one convenient location. All products are quality and availability assured. This bundle of Western Blotting products includes:

  • Acids & Bases
  • Amino Acids
  • Buffered Salt Solutions
  • Precast Gels
  • Substrates
  • General Reagents

Acids & Bases

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H1758 Hydrochloric acid 36.5-38.0%, BioReagent, for molecular biology
    • 216763 Hydrogen peroxide solution contains inhibitor, 30 wt. % in H2O, ACS reagent
    • S8045 Sodium hydroxide BioXtra, ≥98% (acidimetric), pellets (anhydrous)
    • T8657 Trichloroacetic acid for electrophoresis, suitable for fixing solution (for IEF and PAGE gels), ≥99%

    • Acrylamides

    Product #

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    A3553 Acrylamide for electrophoresis, ≥99% (HPLC), powder
    • A8887 Acrylamide suitable for electrophoresis, ≥99%
    • A9099 Acrylamide for molecular biology, ≥99% (HPLC)
    • A4058 Acrylamide solution 40%, for electrophoresis, sterile-filtered
    • M7279 N,N′-Methylenebisacrylamide powder, for molecular biology, for electrophoresis, ≥99.5%
    • M2022 N,N′-Methylenebisacrylamide for molecular biology, ≥98%

    • Amino Acids

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    A8199 N-Acetyl-L-cysteine BioXtra, ≥99% (TLC)
    • G6529 L-Glutathione reduced BioXtra, ≥98.0%
    • G8898 Glycine for electrophoresis, ≥99%
    • L8912 L-Leucine from non-animal source, meets EP, JP, USP testing specifications, suitable for cell culture, 98.5-101.0%

    • Buffers / Buffers Salts

    Product #

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    B8660 BICINE BioXtra, ≥99% (titration)
    • C6070 CAPS BioXtra, pH 3.0-7.0 (20 °C, 0.5 M in H2O), ≥98.0% NaOH basis (titration)
    • M2933 MES hydrate BioReagent, suitable for cell culture, for molecular biology, ≥99.5%
    • M3058 MES sodium salt BioReagent, suitable for cell culture, for molecular biology
    • M1317 MES solution 1 M, BioReagent, for molecular biology, suitable for cell culture
    • M3183 MOPS Biotechnology Performance Certified, cell culture tested, ≥99.5% (titration)
    • M9024 MOPS sodium salt BioReagent, suitable for cell culture, ≥99.5% (titration)
    • M1442 MOPS solution BioReagent, 1 M, for molecular biology, suitable for cell culture
    • P5493 Phosphate buffered saline 10× concentrate, BioReagent, suitable for cell culture, for molecular biology
    • P3813 Phosphate buffered saline powder, pH 7.4
    • P3563 Phosphate buffered saline pH 7.4, contains TWEEN® 20, dry powder
    • P4809 Phosphate-Citrate Buffer tablet
    • S3014 Sodium chloride for molecular biology, DNase, RNase, and protease, none detected, ≥98% (titration)
    • T5816 Tricine Biotechnology Performance Certified, cell culture tested, ≥99% (titration)
    • T6664 Tris Buffered Saline pH 8.0, powder
    • T8793 Tris Buffered Saline, pH 8.0, with 3% nonfat Milk powder
    • T6789 Tris Buffered Saline, with BSA, pH 8.0 powder
    • T9039 Tris Buffered Saline, with Tween® 20, pH 8.0 powder
    • T4904 Tris-Glycine Buffer 10× Concentrate
    • T5941 Trizma® hydrochloride for molecular biology, Biotechnology Performance Certified, cell culture tested, ≥99.0% (titration)
    • T6066 Trizma® base Biotechnology Performance Certified, meets EP, USP testing specifications, cell culture tested, ≥99.9% (titration)

    • Detergents

    Product #

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    L4390 Sodium dodecyl sulfate for molecular biology, ≥98.5% (GC)
    • L3771 Sodium dodecyl sulfate BioReagent, suitable for electrophoresis, for molecular biology, ≥98.5% (GC)
    • T8787 Triton™ X-100 for molecular biology
    • P9416 TWEEN® 20 for molecular biology, viscous liquid
    • P5927 TWEEN® 20 for electrophoresis, suitable for solubilizing agent of membrane proteins and as a blocking reagent in immunoblotting

    • General Reagents

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    A9647 Albumin from bovine serum lyophilized powder, ≥96% (agarose gel electrophoresis)
    • B4287 Albumin from bovine serum BioReagent, for molecular biology
    • B2518 Albumin, Acetylated from bovine serum lyophilized powder, suitable for (for molecular biology)
    • A2504 6-Aminocaproic acid ≥99% (titration), powder
    • A3678 Ammonium persulfate for molecular biology, for electrophoresis, ≥98%
    • B4639 Biotin powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%
    • D9779 DL-Dithiothreitol for molecular biology, ≥98% (TLC), ≥99% (titration)
    • D9163 DL-Dithiothreitol for electrophoresis, ≥99%
    • E1510 Ethidium bromide solution BioReagent, for molecular biology, 10 mg/mL in H2O
    • E5134 Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration)
    • G4004 Glutaraldehyde solution 25 wt. % in H2O
    • G3272 Guanidine hydrochloride for molecular biology, ≥99%
    • M7154 2-Mercaptoethanol for electrophoresis
    • 494437 Methanol suitable for protein sequencing, BioReagent, ≥99.93%
    • M1770 Methanol BioReagent, suitable for protein sequencing
    • S7920 Sodium fluoride BioXtra, ≥99%
    • T9281 N,N,N′,N′-Tetramethylethylenediamine BioReagent, suitable for electrophoresis, ~99%
    • U6504 Urea for electrophoresis
    • W4502 Water Molecular Biology Reagent
    • W3513 Water Biotechnology Performance Certified
    • W0138 Western Blocker™ Solution for HRP detection systems

    • Labware

    Product #

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    Z340502 Semi-dry blotter Blotter unit

    • Markers

    Product #

    Description

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    C1992 ColorBurst™ Electrophoresis Marker mol wt 8,000-220,000 Da
    • C6210 Color Marker Ultra-low Range (M.W. 1,060-26,600)
    • SDS7B2 Prestained Molecular Weight Marker mol wt 26,600-180,000 Da
    • T0565 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System for Membranes ready to use solution

    • Membranes / Blotting Paper

    Product #

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    Z613657 Whatman® Protran® nitrocellulose membranes Rolls - BA83, pore size 0.2 μm, W × L 30 cm × 3 m
    • Z613630 Whatman® Protran® nitrocellulose membranes Rolls - BA85, pore size 0.45 μm, W × L 30 cm × 3 m
    • Z671088 Whatman® Westran® PVDF membranes Westran Clear Signal, 0.45 μm PVDF blotting membrane, 30 cm x 3 m roll, 1/pk

    • Substrates

    Product #

    Description

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    AEC101 AEC Staining Kit liquid
    • AB0300 Alkaline Phosphatase Blue Membrane Substrate Solution sufficient for 400 mL working substrate
    • AM0100 Alkaline Phosphatase Magenta™ Immunohistochemical Substrate Solution
    • A6926 3-Amino-9-ethylcarbazole tablet
    • 90101 Ampliflu™ Red for fluorescence, ≥98.0% (HPLC)
    • B3804 BCIP®/NBT-Blue Liquid Substrate System for Membranes alkaline phosphatase substrate
    • B1911 BCIP®/NBT Liquid Substrate System ready to use solution
    • B3679 BCIP®/NBT-Purple Liquid Substrate System for Membranes ready to use alkaline phosphatase substrate
    • B6404 BCIP®/NBT solution, premixed
    • B6149 5-Bromo-4-chloro-3-indolyl phosphate disodium salt ≥98% (HPLC)
    • B8503 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt ≥99% (HPLC)
    • B0274 5-Bromo-4-chloro-3-indolyl phosphate p-toluidine salt tablet
    • C0712 CDP-Star® Chemiluminescent Substrate 0.25 mM in H2O
    • CPS160 Chemiluminescent Peroxidase Substrate-1 sufficient for 60 mL substrate
    • CPS1120 Chemiluminescent Peroxidase Substrate-1 sufficient for 120 mL substrate
    • CPS3100 Chemiluminescent Peroxidase Substrate-3 sufficient for 100 mL substrate
    • C6788 4-Chloro-1-naphthol tablet
    • C8890 4-Chloro-1-naphthol crystalline
    • D5905 3,3′-Diaminobenzidine tetrahydrochloride tablet, 10 mg substrate per tablet
    • D6815 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride for Membrane Applications
    • D7304 3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride
    • F8764 Fast Red TR Salt hemi(zinc chloride) salt
    • N8518 Naphthol AS-TR phosphate disodium salt tablet, 4 mg substrate per tablet
    • N5514 Nitro Blue Tetrazolium tablet
    • B5655 SIGMAFAST™ BCIP®/NBT tablet
    • D0426 SIGMAFAST™ DAB with Metal Enhancer tablet
    • D4168 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 1 mL
    • D4293 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL
    • D4418 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL
    • F4648 SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 1 mL
    • F4523 SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 10 mL
    • T2885 3,3′,5,5′-Tetramethylbenzidine ≥98% (TLC)
    • T8768 3,3′,5,5′-Tetramethylbenzidine dihydrochloride hydrate powder, ≥98%