Life Science Reagents for Immunohistochemistry (IHC)

Find all the products you need to carry out successful Immunohistochemistry (IHC) in this one convenient location. All products are quality and availability assured. This bundle of Immunohistochemistry products includes:
  • Tissue Processing
  • Organic Solvents
  • Buffers
  • Blocking Sera and Reagents
  • Substrates for Alkaline Phosphatase
  • Substrates for Peroxidase
  • Avidin-Biotin Complex Reagents
  • Antibody Labeling
  • Antibody and Immunoassay Stabilizers
  • Antigen Retrieval Solutions
  • Routine Histology Stains
  • Additional Reagents
  • Lab Accessories

Prestige Antibodies® powered by Atlas Antibodies have 500+ publicly available immunohistological images for each antibody. For more information on Prestige Antibodies and IHC,
click here.

Additional Reagents

Product #

Description

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A3648 (3-Aminopropyl)triethoxysilane ≥98%
    • F4680 Fluoromount™ Aqueous Mounting Medium for use with fluorescent dye-stained tissues
    • F6182 Fluoroshield™ histology mounting medium
    • F6057 Fluoroshield™ with DAPI histology mounting medium
    • F6937 Fluoroshield™ with 1,4-Diazabicyclo[2.2.2]octane New
    • F5932 Fluoroshield™ with PI histology mounting medium
    • F6812 Fluoroshield™ with PPD New
    • GG1 Glycerol Gelatin aqueous slide mounting medium
    • I1161 ImmunoHistoMount™
    • O8015 Organo/Limonene Mount™
    • P8920 Poly-L-lysine solution 0.1 % (w/v) in H2O
    • P5147 Protease from Streptomyces griseus Type XIV, ≥3.5 units/mg solid, powder
    • T7168 Trypsin from porcine pancreas tablet, 1 mg tablet

    • Antibody and Immunoassay Stabilizers

    Product #

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    A4955 Alkaline Phosphatase Stabilizing Buffer liquid
    • 76696 BioStab Alkaline Phosphatase Conjugate Stabilizer
    • 55514 BioStab Antibody Stabilizer
    • 93859 BioStab immunoassay stabilizer

    • Antibody Labeling

    Product #

    Description

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    BTAG BiotinTag™ Micro Biotinylation Kit
    • FITC1 FluoroTag™ FITC Conjugation Kit
    • BK101 ImmunoProbe™ Biotinylation Kit

    • Antigen Retrieval Solutions

    Antigen retrieval solutions improve the response of Immunohistothchemistry response by increasing the accessability of antibodies to tissue antigens. Processing formalin-fixed paraffin-embedded (FFPE) tissue sections with Antigen Retrieval solutions prior to application of antibodies can enhance immunostaining for low-expression proteins. Staining protocols can be optimized to reduce antibody concentration or incubation time.

    Product #

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    C9999 Citrate Buffer, pH 6.0, 10X, Antigen Retriever
    • E1161 EDTA Buffer, pH 8.5, 10X, Antigen Retriever
    • R2283 Pepsin Reagent, Ready to Use, Antigen Retriever
    • T6455 Tris-HCl Buffer, pH 10, 10X, Antigen Retriever

    • Avidin-Biotin Complex Reagents

    Product #

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    E2636 ExtrAvidin® –Alkaline Phosphatase buffered aqueous solution
    • E2886 ExtrAvidin®−Peroxidase buffered aqueous solution
    • EXTRA3 ExtrAvidin® Peroxidase Staining Kit for rabbit antibodies antibody produced in mouse
    • EXTRA2 Mouse ExtrAvidin® Peroxidase Staining Kit antibody produced in goat

    • Blocking Sera and Reagents

    Product #

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    A9647 Albumin from bovine serum lyophilized powder, ≥96% (agarose gel electrophoresis)
    • B4501 Biotin ≥99% (TLC), lyophilized powder
    • B8655 Bovine serum
    • G9023 Goat serum
    • H0146 Horse serum liquid
    • 216763 Hydrogen peroxide solution contains inhibitor, 30 wt. % in H2O, ACS reagent
    • H1009 Hydrogen peroxide solution 30 % (w/w) in H2O, contains stabilizer
    • M5905 Mouse serum
    • R9133 Rabbit serum
    • R9759 Rat serum
    • S3772 Sheep serum
    • L9756 (−)-Tetramisole hydrochloride ≥99% (GC)

    • Buffers

    Product #

    Description

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    18995 BioStab Peroxidase Conjugate Stabilizer
    • ED4SS Ethylenediaminetetraacetic acid tetrasodium salt dihydrate 99.0-102.0% (titration)
    • P9209 Peroxidase Stabilizing Buffer liquid
    • P3813 Phosphate buffered saline powder, pH 7.4
    • P4417 Phosphate buffered saline tablet
    • T5030 Tris Buffered Saline tablet
    • T6664 Tris Buffered Saline pH 8.0, powder
    • 91414 Tris buffered saline with Tween® 20 BioUltra, tablets (for 500 mL), pH 7.6
    • T9039 Tris Buffered Saline, with Tween® 20, pH 8.0 powder
    • T1503 Trizma® base Primary Standard and Buffer, ≥99.9% (titration), crystalline
    • P1379 TWEEN® 20 viscous liquid

    • Lab Accessories

    Product #

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    C9802 Cover glasses size 22 mm × 22 mm
    • C7931 Cover glasses size 24 mm × 40 mm
    • C8181 Cover glasses size 24 mm × 50 mm
    • C9056 Cover glasses size 24 mm × 60 mm
    • Z265985 Dissecting scissors L 5 1/2 in., Mayo, straight
    • F4267 Dressing tissue forceps L 6 in.
    • Z252972 Enviro-Safe® thermometer -20-150 °C temperature, immersion level, 76 mm
    • H6644 Humidity chamber overall size 9 in. × 4 in., trays size 8 in. × 2 1/2 in. , with lid
    • Z380253 Jars Nalgene® style 2118 capacity 500 mL
    • Z188956 Kimwipes® disposable wipers L × W 4 1/2 in. × 8 1/2 in.
    • Z377821 PAP pen for immunostaining 5 mm tip width
    • Z672548 PAP pen for immunostaining 2 mm tip width
    • P0425 Poly-Prep Slides poly-L-lysine coated glass slides
    • S5641 Scienceware® Coplin staining jar with screw cap high-density polyethylene
    • Z648205 Securline lab marker II lab marker, for use on plastic embedding cassettes and frosted end of microscope slides, black, 5/cs
    • SL2 Sigmacote® siliconizing reagent for glass and other surfaces
    • S5769 Sigma lab markers Extra-Fine tip, black
    • S4651 Silane-Prep Slides glass slides coated with silane (aminoalkylsilane).
    • S8400 Slides, microscope Frosted one end, size 25 mm × 75 mm
    • S9027 Slides, microscope Opaque (white), size 25 mm × 75 mm
    • S8902 Slides, microscope Plain, size 25 mm × 75 mm
    • Z648191 STATMARK permanent marker for cassette & slide marking pkg of 12 ea
    • Z672122 Tissue processing/embedding cassettes with lid pkg of 500 ea
    • Z719137 Wash Bottle capacity 500 mL (16 oz)
    • S5516 Wheaton Coplin staining jars 5 slide jar with ground glass cover
    • S5766 Wheaton Coplin staining jars 5 slide jar with plastic screw cap

    • Organic Solvents

    Product #

    Description

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    534064 Acetone histological grade, ≥99.5%
    • M1775 Methanol Absolute - Acetone free
    • R8382 Reagent Alcohol used for histology tissue preparation
    • R3154 Reagent Alcohol, 70% used for histology tissue preparation
    • R3279 Reagent Alcohol, 80% used for histology tissue preparation
    • R3404 Reagent Alcohol, 95% Used for histology tissue preparation
    • 247642 Xylenes ACS reagent, ≥98.5% xylenes + ethylbenzene basis
    • 534056 Xylenes histological grade
    • A5597 Xylene Substitute

    • Routine Histology Stains

    Product #

    Description

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    A3429 Differentiation Solution
    • A3179 Differentiation Solution
    • GHS116 Hematoxylin Solution, Gill No. 1
    • GHS132 Hematoxylin Solution, Gill No. 1
    • GHS1128 Hematoxylin Solution, Gill No. 1
    • GHS216 Hematoxylin Solution, Gill No. 2
    • GHS232 Hematoxylin Solution, Gill No. 2
    • GHS280 Hematoxylin Solution, Gill No. 2
    • GHS2128 Hematoxylin Solution, Gill No. 2
    • MHS16 Hematoxylin Solution, Mayer’s
    • MHS32 Hematoxylin Solution, Mayer’s
    • MHS80 Hematoxylin Solution, Mayer’s
    • MHS128 Hematoxylin Solution, Mayer’s
    • MHS1 Hematoxylin Solution, Mayer’s

    • Substrates for Alkaline Phosphatase

    Product #

    Description

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    B1911 BCIP®/NBT Liquid Substrate System ready to use solution
    • B5655 SIGMAFAST™ BCIP®/NBT tablet
    • F4648 SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 1 mL
    • F4523 SIGMAFAST™ Fast Red TR/Naphthol AS-MX Tablets tablet, To prepare 10 mL

    • Substrates for Peroxidase

    Product #

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    AEC101 AEC Staining Kit liquid
    • D3939 3,3′-Diaminobenzidine (DAB) Enhanced Liquid Substrate System tetrahydrochloride for Immunohistology
    • D7304 3,3′-Diaminobenzidine (DAB) Liquid Substrate System tetrahydrochloride
    • D0426 SIGMAFAST™ DAB with Metal Enhancer tablet
    • D4168 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 1 mL
    • D4293 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 5 mL
    • D4418 SIGMAFAST™ 3,3′-Diaminobenzidine tablets tablet, To prepare 15 mL

    • Tissue Processing

    Product #

    Description

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    HT101128 Bouin’s solution
    • HT10132 Bouin’s solution
    • D0818 Decalcifying Solution-Lite aqueous solution, bp 208 °F
    • D3565 Declere solution 20× concentrate
    • 45348 Epoxy embedding medium, accelerator ≥95% (NT)
    • 45359 Epoxy Embedding Medium kit
    • A5472 Formalin Free Fixative, Accustain™
    • F5554 Formalin, 10%, Neutral Buffered, Wintergreen Scented
    • F5304 Formalin, 10%, Neutral Buffered with 0.03% Eosin
    • FN0010 Formalin Neutralizer Kit
    • HT501128 Formalin solution, neutral buffered, 10% histological tissue fixative
    • HT501320 Formalin solution, neutral buffered, 10% histological tissue fixative
    • HT501640 Formalin solution, neutral buffered, 10% histological tissue fixative
    • G5882 Glutaraldehyde solution Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
    • H2904 HistoChoice® Tissue Fixative
    • 158127 Paraformaldehyde powder, 95%
    • P6148 Paraformaldehyde reagent grade, crystalline
    • P3558 Paraplast® for tissue embedding
    • P3683 Paraplast Plus® for tissue embedding
    • P3808 Paraplast X-TRA® for tissue embedding
    • P6744 Picric acid solution 1.3% in H2O (saturated)
    • S4521 Saponin from quillaja bark Sapogenin content 20-35 %
    • S1878 Sodium (meta)periodate ≥99.0%
    • A6330 Tissue Embedding Medium (Paraplast® Regular)
    • X100 Triton™ X-100 laboratory grade
    • Z2902 Zinc Formalin Fixative