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 2'Fluoro Phosphoramidites

Proligo® Reagents

Product Breadth of Proligo Reagents
 Phosphoramidites:
Pharmadite®
DNA Phosphoramidites
RNA Phosphoramidites
2'O-Methyl RNA Phosphoramidites
2'Fluoro Phosphoramidites
DMT-2'Fluoro Phosphoramidites
5'-NPPOC Phosphoramidites
 Fast Deprotection Chemistries:
DNA
RNA
 Supports:
Controlled Pore Glass (CPG)
Columns
 Solvents:
Liquid Reagents
Activator 42™
 Modifications:
Fluorescein Phosphoramidite
Biotin Phosphoramidite
Phosphate-ON Phosphoramidite
Non Standard Nucleosides
6-FAM
 Linkers:
Amino Linkers
ssH Linker
Product Breadth of Proligo Reagents

2’Fluoro Phosphoramidites are used to synthesize oligonucleotides that are more thermally stable and provide increased nuclease resistance.   View Structures:

DMT-2'Fluoro-dC(ac) Amidite

DMT-2'Fluoro-dU Amidite
 
Key Features of 2'Fluoro Phosphoramidites
Can be employed together with DNA or RNA phosphoramidites
Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA for 10 minutes at 65°C
Synthesis of 2'Fluoro oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 3 minutes compared to 90 seconds for DNA monomers)
Lot-to-lot consistent high purity and performance
Manufactured under a certified ISO 9001 quality system
 

Analytical Specifications Test 2'Fluoro-dC(ac) 2'Fluoro-dU
HPLC Purity ≤98.0% ≤98.0%
31P-NMR ≤99.0% ≤99.0%

Phosphoramidites
Product Information
Product No. Description Unit
C213281-01 DMT-2'Fluoro-dC(ac) Amidite - PerkinElmer 8900 & Polygen 1 x 0.25g
C213231-01 DMT-2'Fluoro-dC(ac) Amidite, ABI 1 x 0.25g
C213231-01 DMT-2'Fluoro-dC(ac) Amidite, ABI 1 x 1g
U211281-01 DMT-2'Fluoro-dU Amidite - PerkinElmer 8900 & Polygen 1 x 0.25g
U211231-01 DMT-2'Fluoro-dU Amidite, ABI 1 x 0.25g
U211231-01 DMT-2'Fluoro-dU Amidite, ABI 1 x 1g

Enquire about delivery dates of these specialty amidites.

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