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2'O-Methyl RNA Phosphoramidites Proligo® Reagents Product Breadth of Proligo Reagents  Phosphoramidites: Pharmadite® DNA Phosphoramidites RNA Phosphoramidites 2'O-Methyl RNA Phosphoramidites 2'Fluoro Phosphoramidites DMT-2'Fluoro Phosphoramidites 5'-NPPOC Phosphoramidites  Fast Deprotection Chemistries: DNA RNA  Supports: Controlled Pore Glass (CPG) Columns  Solvents: Liquid Reagents Activator 42™  Modifications: Fluorescein Phosphoramidite Biotin Phosphoramidite Phosphate-ON Phosphoramidite Non Standard Nucleosides 6-FAM  Linkers: Amino Linkers ssH Linker Product Breadth of Proligo Reagents 2’O-Methyl RNA is a nucleic acid analogue that is characterized by the exceptional hybridization properties that it imparts with complimentary DNA or RNA, as well as increased stability against enzymatic degradation compared to natural nucleic acids.             Download Literature:   •  User Instructions 2'O-Methyl Phosphoramidites (754k pdf)   View Structure:   •  2'O-Methyl-rA(bz) Phosphoramidite   •  2'O-Methyl-rC(tac) Phosphoramidite   •  2'O-Methyl-rG(ib) Phosphoramidite   •  2'O-Methyl-rU Phosphoramidite   •  5-Methyl-dC(ac) Phosphoramidite The unique combination of properties of 2’O-Methyl RNA has found widespread use in the fields of: Diagnostic probes Aptamer and ribozyme development Mixed 2’O-Methyl-RNA/DNA antisense molecules 2’O-Methyl RNA nucleoside can be advantageously incorporated in nucleic acid probes with RNA or DNA for in-vivo or in-vitro applications to convey nuclease resistance.   Key features of 2’O-Methyl RNA Phosphoramidites High yield of oligonucleotides Compatible with DNA synthesis Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides Recommended deprotection conditions are 8 hours at 55°C using concentrated ammonia solution, or with AMA (concentrated ammonia/40% aqueous methylamine I/I, v/v) for 10 minutes at 65°C Purification and other downstream processing of fully modified 2’O-Methyl RNA oligonucleotides are simpler than in the case of RNA, as no special precautions are required to provide protection against nucleolytic degradation Synthesis of 2’O-Methyl RNA oligonucleotides is similar to standard DNA synthesis, but requires an elongated coupling time (recommended is 6 minutes compared to 90 seconds for DNA monomers) 2'O Methyl RNA Phosphoramidites Compatible with Expedite™ Instruments Product No. Description Unit A211181-01 DMT-2′-O-Me-rA(bz) amidite 1 x 0.5g A212181-01 DMT-2’O-Me-rA(tac) Amidite 1 x 0.5g C212181-01 DMT-2′-O-Me-rC(tac) amidite 1 x 0.5g G211181-01 DMT-2′-O-Me-rG(ib) amidite 1 x 0.5g G212181-01 DMT-2′-O-Me-rG(tac) amidite 1 x 0.5g U211181-01 DMT-2′-O-Me-rU amidite 1 x 0.5g Compatible with ABI™ Instruments Product No. Description Unit A211131-01 DMT-2′-O-Me-rA(bz) amidite 1 x 0.5g A212131-01 DMT-2′-O-Me-rA(tac) amidite 1 x 0.5g C212131-01 DMT-2′-O-Me-rC(tac) amidite 1 x 0.5g G211131-01 DMT-2′-O-Me-rG(ib) amidite 1 x 0.5g G212131-01 DMT-2′-O-Me-rG(tac) amidite 1 x 0.5g U211131-01 DMT-2′-O-Me-rU amidite 1 x 0.5g Bulk Quantities Product No. Description Unit A211110-01 DMT-2′-O-Me-rA(bz) amidite 1 x 10g A212110-01 DMT-2′-O-Me-rA(tac) amidite 1 x 10g C212110-01 DMT-2′-O-Me-rC(tac) amidite 1 x 10g G211110-01 DMT-2′-O-Me-rG(ib) amidite 1 x 10g G212110-01 DMT-2′-O-Me-rG(tac) amidite 1 x 10g U211110-01 DMT-2′-O-Me-rU amidite 1 x 10g 2'O Methyl RNA CPG Product No. Description Unit   500 Å, extent of labeling: 30-40 μmol/g A601101-01 2′-O-Methyl-rA(bz)-CPG 1 x 1g   500 Å, extent of labeling: 30-40 μmol/g C602101-01 2′-O-Methyl-rC(tac)-CPG 1 x 1g   500 Å, extent of labeling: 30-40 μmol/g G601101-01 2′-O-Methyl-rG(ib)-CPG 1 x 1g   500 Å, extent of labeling: 30-40 μmol/g U601101-01 2′-O-Methyl-rU-CPG 1 x 1g 2'O Methyl RNA Columns Compatible with Expedite™ Instruments Product No. Description Unit   500 Å, extent of labeling: 1 μmol A631104-01 2′-O-Me-rA(bz)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol C632104-01 2′-O-Me-rC(tac)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol G631104-01 2′-O-Me-rG(ib)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol U631104-01 2′-O-Me-rU-CPG column 1 x 4 Compatible with ABI™ Instruments Product No. Description Unit   500 Å, extent of labeling: 1 μmol A661104-01 2′-O-Me-rA(bz)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol C662104-01 2′-O-Me-rC(tac)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol G661104-01 2′-O-Me-rG(ib)-CPG column 1 x 4   500 Å, extent of labeling: 1 μmol U661104-01 2′-O-Me-rU-CPG column 1 x 4 Custom Products Bulk packaging up to multiple kg per container and customized packaging with alternative quantities of amidites are available upon request. 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