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Biotin Phosphoramidite
| Product Breadth of Proligo Reagents |
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| Product Breadth of Proligo Reagents |
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| Biotin-labeled oligonucleotides have become extremely popular in DNA capture and detection applications, due to their very high sensitivity and strong, specific binding affinity with avidin and streptavidin proteins. Biotin-based detection methods are widely used by researchers. Biotin labeling is compatible with PCR and most hybridization techniques. |
| Key Features of the Biotin Phosphoramidite |
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Behaves like any standard amidite on the DNA synthesizer |
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Readily soluble in acetonitrile |
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Biotin compound has a dimethozytrityl (DMT) — group on the ring structure, which allows coupling-yield determination and trityl-selective purification |
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A long, water-compatible linker arm ensures maximum sensitivity |
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The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required |
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Provides a coupling efficiency of 95% |
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Download Literature:
User Instructions
Biotin Phosphoramidite
(670 Kb PDF)
View Structure:
DMT-Biotin-β-Cyanoethy-lphosphoramidite |
| Labels and Modifications - Biotin Phosphoramidite |
| Compatible with Expedite™ and Polygen® Instruments |
| Product No. |
Description |
Unit |
| M010381-01 |
Biotin amidite |
0.1g |
| M010382-01 |
Biotin amidite |
0.25 g |
| Compatible with ABI™ Instruments |
| M010331-01 |
Biotin amidite |
0.1g |
| M010332-01 |
Biotin amidite |
0.25 g |
| Custom Products |
Phosphoramidites of non-catalogue linkers, reporters or modifiers are offered as custom synthesis products. Please enquire. |
SAFC and SAFC Proligo Reagents are registered trademarks of Sigma-Aldrich Biotechnology, LP.
© 2009 Sigma-Aldrich Biotechnology LP. All rights reserved. Reproduction forbidden without permission.
Other trademarks are the property of their respective owners.
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