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Controlled Pore Glass (Free flow CPG) Proligo® Reagents Product Breadth of Proligo Reagents  Phosphoramidites: Pharmadite® DNA Phosphoramidites RNA Phosphoramidites 2'O-Methyl RNA Phosphoramidites 2'Fluoro Phosphoramidites DMT-2'Fluoro Phosphoramidites 5'-NPPOC Phosphoramidites  Fast Deprotection Chemistries: DNA RNA  Supports: Controlled Pore Glass (CPG) Columns  Solvents: Liquid Reagents Activator 42™  Modifications: Fluorescein Phosphoramidite Biotin Phosphoramidite Phosphate-ON Phosphoramidite Non Standard Nucleosides 6-FAM  Linkers: Amino Linkers ssH Linker Product Breadth of Proligo Reagents Since Merrifield first described solid phase synthesis, researchers have investigated numerous supports for the chemical synthesis of DNA. Controlled Pore Glass (CPG) has proven to be the most accepted standard, with features such as high surface area, efficient mass transfer and chemical inertness. Because the pore density of CPG can be adjusted, the solid support can be tailored to the size of the synthetic oligonucleotide required. Bigger pore size is typically preferable in minimizing steric effects during the synthesis of long oligonucleotides, while smaller pore size fosters improved synthesis consistency. Our process guarantees consistent production of CPGs to specifications designed for DNA synthesis. All products are tested in our Quality Control labs for purity and identity prior to release. SAFC Proligo Reagent’s facility in Hamburg, Germany is ISO 9001 certified.  On this Page DNA CPG Fast deprotection DNA CPG RNA CPG Amino-ON CPG Universal CPG DNA CPG Analytical Parameters of CPG   CPG (UHL) CPG 500Å CPG 1000Å Pore size (nm) 33 50 100 Pore volume (ml/g) 1.3 1.3 1.3 Grain size (µm) 100 - 180 100 – 180 100 - 180 Surface (m2/g) ~100 ~60 ~30 Nucleoside-Loaded CPG   Loading µmol/g CPG 500Å 30-40 CPG 1000Å 25-35 CPG UHL 50-70 CPG UHL 90-110 DNA Controlled Pore Glass Compatible with Expedite™ and Polygen® Instruments Product No. Description Unit   500 Å, extent of labeling: 30-40 μmol/g A301001-01 dA(bz)-CPG 1 g A301010-01 dA(bz)-CPG 10 g   1000 Å, extent of labeling: 25-35 μmol/g A401001-01 dA(bz)-CPG 1 g A401010-01 dA(bz)-CPG 10 g   500 Å, extent of labeling: 30-40 μmol/g C301001-01 dC(bz)-CPG 1 g C301010-01 dC(bz)-CPG 10 g   1000 Å, extent of labeling: 25-35 μmol/g C401001-01 dC(bz)-CPG 1 g C401010-01 dC(bz)-CPG 10 g   500 Å, extent of labeling: 30-40 μmol/g G301001-01 dG(ib)-CPG 1 g G301010-01 dG(ib)-CPG 10 g   1000 Å, extent of labeling: 25-35 μmol/g G401001-01 dG(ib)-CPG 1 g G401010-01 dG(ib)-CPG 10 g   500 Å, extent of labeling: 30-40 μmol/g T301001-01 dT-CPG 1 g T301010-01 dT-CPG 10 g   1000 Å, extent of labeling: 25-35 μmol/gg T401001-01 dT-CPG 1 g T401010-01 dT-CPG 10 g Custom Products Bulk packaging up to multiple kg per container and customized packaging with alternative quantities of amidites are available upon request.   CPG-products can be provided with higher loadings based on UHL-CPG (50-70 µmol/g or 90-110 µmol/g), please enquire. Fast deprotection DNA CPG Fast Deprotection CPG Product No. Description Unit A302001-01 CPG 500Å, dA(tac), 30-40 μmol/g 1 x 1g C302001-01 CPG 500Å, dC(tac), 30-40 μmol/g 1 x 1g G302001-01 CPG 500Å, dG(tac), 30-40 μmol/g 1 x 1g A402001-01 CPG 1000Å, dA(tac), 25-35 μmol/g 1 x 1g C402001-01 CPG 1000Å, dC(tac), 25-35 μmol/g 1 x 1g G402001-01 CPG 1000Å, dG(tac), 25-35 μmol/g 1 x 1g C302010-01 CPG 500Å, dC(tac), 30-40 μmol/g 1 x 10g C402010-01 CPG 500Å, dC(tac), 25-35 μmol/g 1 x 10g G305001-01 CPG 500Å, dG(tac), 30-40 μmol/g 1 x 1g G305010-01 CPG 500Å, dG(tac), 30-40 μmol/g 1 x 10g RNA CPG Fast Deprotection RNA CPG Product No. Description Unit A602001-01 CPG 500Å, rA(tac), 25-35 μmol/g 1 x 1g C602001-01 CPG 500Å, rC(tac), 25-35 μmol/g 1 x 1g G602001-01 CPG 500Å, rG(tac), 25-35 μmol/g 1 x 1g U601001-01 CPG 500Å, rU, 25-35 μmol/g 1 x 1g 2'0-Methyl RNA CPG Product No. Description Unit A601101-01 CPG 500Å 2'O-Methyl-rA(bz), 25-35 μmol/g 1 x 1g C602101-01 CPG 500Å 2'O-Methyl-rC(tac), 25-35 μmol/g 1 x 1g G601101-01 CPG 500Å 2'O-Methyl-rG(ib), 25-35 μmol/g 1 x 1g U601101-01 CPG 500Å 2;O-Methyl-rU, 25-35 μmol/g 1 x 1g Amino-ON CPG Amino-ON CPG can be employed to conjugate biotin, fluorescein or other modifiers and reporter groups to the 3'-end of oligonucleotides or to attach oligonucleotides to surfaces. Amino-ON CPG from SAFC Proligo Reagents combines the advanced features of cleavage and deprotection under mild conditions, and maintenance of the integrity of the 3'-modification with high synthesis yields.             Download Literature:   •  User Instructions Amino-ON CPG (667k pdf)   View Structure:   •  Amino-ON CPG Key Features of 3’-Amine Oligonucleotides Selective conjugation to reporter groups, purification handles or other modifiers 3'-end attachment to electrophilic surfaces Offers protection against exonucleolytic degradation These features enable 3’-amine oligonucleotides to be used in applications such as 3’-labeling with base sensitive dyes; 3’-biotin modifications; dual-labeled probes (eg TaqMan® probes and Molecular beacons); and microarray spotting.   Key Features of Amino-ON CPG Introduces a primary amino group attached to a C6-linker Fully compatible with standard phosphoramidite reagents and synthesis conditions Can be employed together with DNA or RNA phosphoramidites in the same synthesis to produce mixmer oligonucleotides Can be used with DNA, RNA and Locked Nucleic Acid® (LNA®); 5'-oligonucleotide modifications such as fluorescein or biotin; and with the synthesis of thioated DNA oligonucleotides Compatible with dG(dmf) fast deprotection chemistry: the oligonucleotide can be cleaved from the support and deprotected in concentrated ammonia at 55°C in 2 hours Provides all the advantages of homogeneous pore size porous glass supports Standard pore size of 500Å Loading of 30-40 µmol/g Coupling performance of ≥ 99.0% stepwise efficiency DMT-protected in the same way as standard nucleoside-loaded CPG Amino on CPG   500 Å, extent of labeling: 30‑40 μmol/g M020101-01 Amino-ON CPG   Universal CPG Universal CPG from SAFC Proligo Reagents is truly universal as it can be employed in the synthesis of any oligonucleotide sequence instead of conventional nucleoside loaded CPG - whether loaded with adenosine, cytidine, guanosine or thymidine nucleosides. The use of universal CPG releases your resources as the purchase, handling and storage of differentially loaded types of nucleoside-CPG is no longer required. And 3’-end synthesis errors, because of accidental interchange of polymeric supports, are essentially eliminated.             Download Literature:   •  User Instructions Universal CPG (659k pdf)   View Structure:   •  Universal CPG         Key Features of Universal CPG Fully compatible with standard phosphoramidite reagents and synthesis conditions Can be employed in the synthesis of many different types of oligonucleotides: DNA oligonucleotides Phosphorothioates 2’O-Methyl oligonucleotides Locked Nucleic Acid® (LNA®) oligonucleotides Fluorescein, tetrachloro-fluorescein and biotin modifications Particularly useful for high-throughput oligonucleotide synthesis Coupling efficiency of ≥ 99.0% is routinely obtained Convenient cleavage and deprotection Avoids the use of unusual and potentially harmful reagents in the deprotection process e.g. heavy metals, sulfides     Universal CPG Product No. Description Unit   500 Å, extent of labeling: 30-40 μmol/g M301001-01 Universal CPG 1 g M301010-01 Universal CPG 10 g   1000 Å, extent of labeling: 25-35 μmol/g M401001-01 Universal CPG 1 g M401010-01 Universal CPG 10 g SAFC and SAFC Proligo Reagents are registered trademarks of Sigma-Aldrich Biotechnology, LP. ©2009 Sigma-Aldrich Biotechnology LP. All rights reserved. Reproduction forbidden without permission. Other trademarks are the property of their respective owners. To order or speak with a Customer Service Representative, contact your local SAFC office.