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Fluorescein Phosphoramidite Proligo® Reagents Product Breadth of Proligo Reagents  Phosphoramidites: Pharmadite® DNA Phosphoramidites RNA Phosphoramidites 2'O-Methyl RNA Phosphoramidites 2'Fluoro Phosphoramidites DMT-2'Fluoro Phosphoramidites 5'-NPPOC Phosphoramidites  Fast Deprotection Chemistries: DNA RNA  Supports: Controlled Pore Glass (CPG) Columns  Solvents: Liquid Reagents Activator 42™  Modifications: Fluorescein Phosphoramidite Biotin Phosphoramidite Phosphate-ON Phosphoramidite Non Standard Nucleosides 6-FAM  Linkers: Amino Linkers ssH Linker Product Breadth of Proligo Reagents Fluorescein phosphoramidite is composed of a protected fluorescein molecule linked to a ß-cyanoethylphosphoramidite. The fluorescein derivative consists of to isomers derived form 5- and 6- carboxy fluorescein. As a result, two peaks will be seen on a RP-HPLC chromatogram of the synthesis product.             Download Literature:   •  User Instructions Fluorescein Phosphoramidite (656k pdf)   View Structure:   •  Fluorescein-ß- Cyanoethyl phosphoramidite       Key Features of the Fluorescein Phosphoramidite   Readily soluble in acetonitrile   Couples to the 5’ end of the oligonucleotide using standard synthesis protocols Protecting groups on the fluorescein moiety are removed under standard conditions of cleavage and deprotection with concentrated ammonia A detritylation step is not required since the fluorescein phosphoramidite does not contain a dimethoxytrityl (DMT-) group The labeled oligonucleotide is ready for use in most applications after the evaporation of ammonia. Standard procedures can be used if additional purification is required The fluorescein moiety provides a purification handle in RP-HPLC purification     Labels and Modifications - Fluorescein Phosphoramidite Compatible with Expedite™ and Polygen® Instruments Product No. Description Unit M010181-01 Fluorescein amidite 1 x 0.1g Compatible with ABI™ Instruments M010131-01 Fluorescein amidite 1 x 0.1g Custom Products Phosphoramidites of non-catalogue linkers, reporters or modifiers are offered as custom synthesis products. Please enquire. SAFC and SAFC Proligo Reagents are registered trademarks of Sigma-Aldrich Biotechnology, LP. ©2009 Sigma-Aldrich Biotechnology LP. All rights reserved. Reproduction forbidden without permission. Other trademarks are the property of their respective owners. To order or speak with a Customer Service Representative, contact your local SAFC office.