Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.


CompoZr® Zinc Finger Nucleases (ZFNs) for Biological Manufacturing

SAFC recognizes that one of the most time consuming and costly endeavors biopharmaceutical companies face is creating robust, efficient cell lines. Zinc Finger Nuclease (ZFN) technology allows for easy creation of novel CHO cell lines with precise and heritable gene additions, deletions or modifications.

Click on image for larger view.

Figure A. ZFNs are a class of engineered DNA-binding proteins that facilitate targeted editing of the genome by creating double-strand breaks in DNA at user-specified locations. Figure B. Double-strand breaks are important for site-specific mutagenesis in that they stimulate the cell's natural DNA-repair processes, namely homologous recombination (HR) and non-homologous end joining (NHEJ). Using well-established and robust protocols, these cellular processes can be harnessed to generate precisely targeted genomic edits resulting in cell lines, with targeted gene deletions (knockouts), integrations (knock-ins), or modifications.  

Applications of Cell Lines

SAFC has created catalog ZFNs for use in the biopharmaceutical industry. A summary of bioproduction applications using ZFN technology is shown below:

Bioproduction Application
Gene Target Notes
CHOZN Cell Line Availability CompoZr ZFN Availability
Metabolic Selection Glutamine Synthetase (GS) Platform Cell Line; cGMP Banked; Full Documentation & Traceability CHOGS-1VL ZFNGS-1KT
Dihydrofolate Reductase (DHFR) Full Documentation & Traceability CHODHFR-1VL ZFNDHFR-1KT
Glycoengineering CMP-N-acetylneuraminic acid hydroxylase (CMAH) & Alpha-1-3-galactosyltransferase (GGTA-1)
Optimal Glycosylation & Reduce Immunogenicity Yes. Trait Stacked in CHOGS. Contact CHOZN@sial.com for ordering. Yes! We can target any gene! Contact CHOZN@sial.com for ordering. Product number is SAFCZFN-1KT.
Mannosyl (alpha-1,3-)-glycoprotein beta-1,2-N-acetylglucosaminyltransferase (MGAT1) Cell Culture Performance Yes. Trait Stacked in CHOGS. Contact CHOZN@sial.com for ordering.
Cell Culture Performance BCL2-associated X protein (BAX) & BCL2-antagonist/killer 1 (BAK) Enhanced Titer, Growth, Longevity
No
Targeted Integration Determined By End User Consistent, Stable Expression No
Interfering Host Cell Protein Targeted Gene Knockout N/A
Risk Mitigation Viral Resistance, Retroviral Elements N/A
Trait Stacking Selection; Glycosylation; Cell Culture Performance N/A
Product Quality/Stability Sialidation Consistency N/A

ZFN Formats

We offer ZFN reagents in DNA plasmid format as well as mRNA. mRNA allows for transient expression without the ability to integrate into the genome, which can occur using plasmid DNA. Recently, we’ve added ZFNs which are fluorescently tagged! This allows the customer to perform FACS, resulting in an enriched pool.

For ZFN inquiries please email CHOZN@sial.com and a scientist will discuss your project goals with you and propose the best strategy.

View Brochure
Learn more about the CHOZN Expression Platform
Download Technical Bulletin
Research product features
 
Contact SAFC
Please contact us for order, product, or service inquires, quotes, or questions.