Oligo Upload

• Instructions to Order Only One Type of Oligo
• Instructions to Order More Than One Type of Oligo
• Plate Formats
• Ordering/Modification Codes

Instructions to Order Only One Type of Oligo

• Select the product type and file format from the drop down menu
  
  
• Format Your Sequences
  
  
  • MS Excel Format (Tab Delimited): Column A = Oligo Name, Column B = Sequence, Column C = Scale (or Fixed OD), Column D = Purification, Column E = Special Instructions (Optional)
    
    Example:

    My Oligo1	[A488]ACTGTGCATATTTTCCATAGCGT	0.2	HPLC
    My Oligo2	ACTGTGCATACCCATAGCGATTTTTT	0.2	HPLC
    My Oligo3	ACTGTGCATACCATAGCGTATATATAT	0.2	HPLC

    
    
    
  • csv file Format (Comma Separated): Oligo Name, Sequence, Scale, Purification, Special Instructions (Optional)
    
    Example:
    My Oligo1,[A488]ACTGTGCATATTTTCCATAGCGT,0.2,HPLC
    My Oligo2,ACTGTGCATACCCATAGCGATTTTTT,0.2,HPLC
    My Oligo3,ACTGTGCATACCATAGCGTATATATAT,0.2,HPLC
  
  
  
• Define Product Packaging and Format. To find details about plate formats, click here.
  
  
• Items can be added to your shopping cart by clicking Add to Cart.

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Instructions to Order More Than One Type of Oligo

• Select "Defined in Data—Supports multiple types in one upload" from the drop down menu
  
  
• Select file format from the drop down menu
  
  
• Format Your Sequences
  
  
  • MS Excel Format (Tab Delimited): Column A = Oligo Type Code, Column B = Oligo Name, Column C = Sequence, Column D = Scale (or Fixed OD), Column E = Purification, Column F = Special Instructions (Optional)
    
    Example:

    STD	My Oligo1	[A488]ACTGTGCATATTTTCCATAGCGT	0.2	HPLC
    STD	My Oligo2	ACTGTGCATACCCATAGCGATTTTTT	0.2	HPLC
    STD	My Oligo3	ACTGTGCATACCATAGCGTATATATAT	0.2	HPLC
    BBP	BigBand Primer 1	ACTGTGCATACCATAGCGTATATATAT	0.05	DST

    
    
    
  • csv file Format (Comma Separated): Oligo Type Code, Oligo Name, Sequence, Scale, Purification, Special Instructions (Optional)
    
    Example:
    STD,My Oligo1,[A488]ACTGTGCATATTTTCCATAGCGT,0.2,HPLC
    STD,My Oligo2,ACTGTGCATACCCATAGCGATTTTTT,0.2,HPLC
    STD,My Oligo3,ACTGTGCATACCATAGCGTATATATAT,0.2,HPLC
    BBP,My BigBand,ACTGTGCATACCATAGCGTATATATAT,0.05,DST
  
  
  
• Define Product Packaging and Format. To find details about plate formats, click here.
  
  
• Items can be added to your shopping cart by clicking Add to Cart.

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Plate Formats

• MS Excel Format: Column A = Oligo Name, Column B = Sequence, Column C = Scale (or Fixed OD), Column D = Purification, Column E = Plate Name (Optional)
  
  
• csv file Format (Comma Separated): Oligo Name, Sequence, Scale, Purification, Plate Name (Optional)

96-Well Plate(s), 8-Channel

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96-Well Plate(s), 12-Channel

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Ordering Codes

Product Type Description	Oligo Type Code
DNA Oligos - modified and non-modified	STD
Dual labeled fluorogenic probe	DLFP
Molecular Beacon	MB
BigBand™ Primer	BBP
F@ST	F@STSTOLIGO
E@SY	E@SYOLIGO
WellRED	WELLRED
Pure and Simple Primer	PSP

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Scale of Synthesis Description	Scale Code
0.025 µmol	0.025
0.05 µmol	0.05
0.2 µmol	0.2
1 µmol	1
10 µmol	10
15 µmol	15

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Fixed OD Description (Labeled Probes & Beacons)	Fixed OD Code
1 OD	1
3 OD	3
5 OD	5
10 OD	10
50 OD	50
100 OD	100

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Purification Description	Purification Code
Deprotected and Desalted	DST
Reverse Phase Cartridge Purification	RP1
HPLC	HPLC
PAGE	PAGE

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5' Modification Description	5' Mod Code
Alexa A488	[A488]
Alexa A546	[A546]
5'Amine C12	[AmC12]
Amine C6 TFA (Desalt, PAGE)	[AmC6F]
5' Amino C6 MMT (RP1, HPLC)	[AmC6T]
Biotin	[Btn]
Biotin TEG	[BtnTg]
5-Bromo dC	[5BrdC]
5-Bromo dU	[5BrdU]
8-Bromo dA	[8BrdA]
8-Bromo dG	[8BrdG]
Carboxy-Rhodamine 6G	[CRh6G]
CY 3	[Cy3]
CY 5	[Cy5]
CY 5.5	[Cy55]
deoxyInosine	I
deoxyUridine	U
Digoxigenin	[DIG]
6-FAM	[6FAM]
Fluorescein	[Flc]
HEX	[HEX]
JOE	[JOE]
5' Phosphorylation	[Phos]
ROX	[ROX]
TAMRA	[TAM]
TET	[TET]
Texas Red	[TxRd]
Thiol C6 S-S	[ThiC6]
WellRED D2-PA	[D2-PA]
WellRED D3-PA	[D3-PA]
WellRED D4-PA	[D4-PA]
SPECIAL (Please define)	[SPECIAL]

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Base/IUB Redundancy Description	Base/IUB Code
A (adenine)	A
C (cytosine)	C
G (guanine)	G
T (thymine)	T
Phosphorothioate linkages	*
2'O-Methyl RNA	[mA], [mG], [mC], [mU]
LNA Bases	[+A], [+G], [+C], [+T]
DNA bases within RNA oligos	[dA], [dC], [dG], [dT]
B = G+T+C	B
D = G+A+T	D
H = A+T+C	H
K = G+T	K
M = A+C	M
N = A+C+G+T	N
R = A+G	R
S = G+C	S
V = A+C+G	V
W = A+T	W
Y = C+T	Y

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Internal Modification Description	Internal Mod Code
Amino C2 dT	[AmC2T]
Amino C6 dT	[AmC6T]
Biotin TEG	[BtnTg]
Biotin dT	[BtndT]
5-Bromo dC	[5BrdC]
5-Bromo dU	[5BrdU]
8-Bromo dA	[8BrdA]
8-Bromo dG	[8BrdG]
deoxylnosine	I
deoyxUridine	U
Fluorescein dT	[FlcdT]
5 Methyl dC	[5MedC]
SPECIAL (Please define)	[SPECIAL]

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3' Modification Description	3' Mod Code
Alexa A488	[A488]
Alexa A546	[A546]
3'Amine C3	[AmC3]
3'Amine C7	[AmC7]
Biotin TEG	[BtnTg]
CY3	[Cy3]
CY5	[Cy5]
deoxylnosine	I
deoxyUridine	U
Digoxigenin	[DIG]
Fluorescein	[Flc]
JOE	[JOE]
3' Phosphorylation	[Phos]
ROX	[ROX]
Spacer C3	[SpcC3]
TAMRA	[TAM]
Texas Red	[TxRd]
Thiol C3	[ThiC3]
SPECIAL (Please define)	[SPECIAL]

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