Muse Publications

 October 2017


HSP90 inhibitor (NVP-AUY922) enhances the anti-cancer effect of BCL-2 inhibitor (ABT-737) in Small cell lung cancer expressing BCL-2 
Original Research Article
Cancer Letters, Available online 4 October 2017, Pages

Hannah Yang, Mi-Hee Lee, Intae Park, Hanwool Jeon, Junyoung Choi, Seyoung Seo, Sang-We Kim, Gou Young Koh, Kang-Seo Park, Dae Ho Lee
Cordycepin induces apoptotic cell death of human brain cancer through the modulation of autophagy
Original Research Article
Toxicology in Vitro, Available online 4 October 2017, Pages

Nipha Chaicharoenaudomrung, Thiranut Jaroonwitchawan, Parinya Noisa

Graphical abstract
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 September 2017


Tuning the structure of aminoferrocene-based anticancer prodrugs to prevent their aggregation in aqueous solution
Original Research Article
Journal of Inorganic Biochemistry, Available online 15 September 2017, Pages

Steffen Daum, Svetlana Babiy, Helen Konovalova, Walter Hofer, Alexander Shtemenko, Natalia Shtemenko, Christina Janko, Christoph Alexiou, Andriy Mokhir

Graphical abstract
Substitution of N-benzyl for an N-propargyl residue in the anticancer prodrug 4-(N-benzyl-N-ferrocenylaminocarbonyloxymethyl)phenylboronic acid pinacol ester led to reduction of its lipophilicity, improvement of its water solubility, decrease of its propensity towards aggregation and dramatic increase of its ROS-generating properties without any negative effect on its anticancer activity.
Globularifolin exerts anticancer effects on glioma U87 cells through inhibition of Akt/mTOR and MEK/ERK signaling pathways in vitro and inhibits tumor growth in vivo 
Original Research Article
Biochimie, Available online 11 September 2017, Pages

Yunhu Yu, Xiaohong Fu, Qishan Ran, Kaihua Yang, Yuanchao Wen, Hang Li, Fei Wang

Graphical abstract
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Selective expression of the transcription elongation factor ELL3 in B cells prior to ELL2 drives proliferation and survival 
Original Research Article
Molecular Immunology, Volume 91, November 2017, Pages 8-16

Lou-Ella M.M. Alexander, January Watters, Jessica A. Reusch, Michelle Maurin, Brook S. Nepon-Sixt, Katerina Vrzalikova, Mark G. Alexandrow, Paul G. Murray, Kenneth L. Wright
Harvesting of Dunaliella salina by membrane filtration at pilot scale   
Original Research Article
Separation and Purification Technology, Available online 1 September 2017, Pages

Joana Monte, Marta Sá, Cláudia F. Galinha, Luís Costa, Herre Hoekstra, Carla Brazinha, João G. Crespo
Silencing of the pentose phosphate pathway genes influences DNA replication in human fibroblasts  
Gene, Available online 5 September 2017, Pages

Karolina Fornalewicz, Aneta Wieczorek, Grzegorz Węgrzyn, Robert Łyżeń


 August 2017


Reduced levels of methyltransferase DNMT2 sensitize human fibroblasts to oxidative stress and DNA damage that is accompanied by changes in proliferation-related miRNA expression   
Original Research Article
Redox Biology, Available online 18 August 2017, Pages
Anna Lewinska, Jagoda Adamczyk-Grochala, Ewa Kwasniewicz, Anna Deregowska, Ewelina Semik, Tomasz Zabek, Maciej Wnuk
Mitochondrial Dysfunction and Inflammatory Response in the Cytotoxicity of NR8383 Macrophages Induced by Fine Particulate Matter   
Original Research Article
Environmental Toxicology and Pharmacology, Available online 29 July 2017, Pages
Qi Xiong, Qin Ru, Lin Chen, Xiang Tian, Chaoying Li


 July 2017


Antitumor potential of 1-thiocarbamoyl-3,5-diaryl-4,5-dihydro-1H-pyrazoles in human bladder cancer cells   
Original Research Article
Biomedicine & Pharmacotherapy, Volume 94, October 2017, Pages 37-46
Josiane Weber Tessmann, Julieti Buss, Karine Rech Begnini, Lucas Moraes Berneira, Favero Reisdorfer Paula, Claudio Martin Pereira de Pereira, Tiago Collares, Fabiana Kömmling Seixas
In vitro genotoxicity and cytotoxicity of polydopamine-coated magnetic nanostructures   
Original Research Article
Toxicology in Vitro, Volume 44, October 2017, Pages 256-265
Anna Woźniak, Magdalena Walawender, Dominika Tempka, Emerson Coy, Karol Załęski, Bartosz F. Grześkowiak, Radosław Mrówczyński
In vitro studies of the anticancer action of Tectaria cicutaria in human cancer cell lines: G0/G1 p53-associated cell cycle arrest-Part I   
Original Research Article
Journal of Traditional and Complementary Medicine, Available online 26 July 2017, Pages
Preeti Gajendra Karade, Namdeo Ramhari Jadhav

Graphical abstract

Aptamer-Based Electrochemical Biosensor for Highly Sensitive and Selective Malaria Detection with Adjustable Dynamic Response Range and Reusability   
Original Research Article
Sensors and Actuators B: Chemical, Available online 21 July 2017, Pages
Gabriela Figueroa Miranda, Lingyan Feng, Simon Chi-Chin Shiu, Roderick Marshall Dirkzwager, Yee-Wai Cheung, Julian Alexander Tanner, Michael Josef Schöning, Andreas Offenhäusser, Dirk Mayer
The nitroxide 4-methoxy TEMPO inhibits neutrophil-stimulated kinase activation in H9c2 cardiomyocytes   
Original Research Article
Archives of Biochemistry and Biophysics, Available online 5 July 2017, Pages
B. Chami, G. Jeong, A. Varda, A.-M. Maw, H.-B. Kim, G.M. Fong, M. Simone, B.S. Rayner, X.-S. Wang, J.M. Dennis, P.K. Witting


 May 2017


2D Fluorescence spectroscopy for monitoring Dunaliella salina concentration and integrity during membrane harvesting
Original Research Article
Algal Research, Volume 24, Part A, June 2017, Pages 325-332
Marta Sá, Joana Monte, Carla Brazinha, Claudia F Galinha, João G Crespo
Klotho protects human monocytes from LPS-induced immune impairment associated with immunosenescent-like phenotype 
Original Research Article
Molecular and Cellular Endocrinology, Available online 3 May 2017, Pages
Jennifer Mytych, Maria Romerowicz-Misielak, Marek Koziorowski
Metabotropic glutamate receptor 5 mediates the suppressive effect of 6-OHDA-induced model of Parkinson’s disease on liver cancer
Original Research Article
Pharmacological Research, Available online 26 April 2017, Pages
Shao-Song Xi, Xiao-Xu Bai, Li Gu, Li-Hui Bao, Hui-Min Yang, Wei An, Xiao-Min Wang, Hong Zhang
Melicope ptelefolia leaf extracts exhibit antioxidant activity and exert anti-proliferative effect with apoptosis induction on four different cancer cell lines


BMC Complementary and Alternative Medicine

Mohammad Faujul Kabir, Johari Mohd Ali, Mitra Abolmaesoomi and Onn Haji Hashim

Melicope ptelefolia is a well-known herb in a number of Asian countries. It is often used as vegetable salad and traditional medicine to address various ailments. However, not many studies have been currently done to evaluate the medicinal benefits of M. ptelefolia (MP). The present study reports antioxidant, anti-proliferative, and apoptosis induction activities of MP leaf extracts.

Young MP leaves were dried, powdered and extracted sequentially using hexane (HX), ethyl acetate (EA), methanol (MeOH) and water (W). Antioxidant activity was evaluated using ferric reducing antioxidant power (FRAP), 2,2′-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) and 1,1-Diphenyl-2-picryl-hydrazyl (DPPH) radicals scavenging and cellular antioxidant activity (CAA) assays. Anti-proliferative activity was evaluated through cell viability assay, using the following four human cancer cell lines: breast (HCC1937, MDA-MB-231), colorectal (HCT116) and liver (HepG2). The anti-proliferative activity was further confirmed through cell cycle and apoptosis assays, including annexin-V/7-aminoactinomycin D staining and measurements of caspase enzymes activation and inhibition.

Overall, MP-HX extract exhibited the highest antioxidant potential, with IC50 values of 267.73 ± 5.58 and 327.40 ± 3.80 μg/mL for ABTS and DPPH radical-scavenging assays, respectively. MP-HX demonstrated the highest CAA activity in Hs27 cells, with EC50 of 11.30 ± 0.68 μg/mL, while MP-EA showed EC50 value of 37.32 ± 0.68 μg/mL. MP-HX and MP-EA showed promising anti-proliferative activity towards the four cancer cell lines, with IC50 values that were mostly below 100 μg/mL. MP-HX showed the most notable anti-proliferative activity against MDA-MB-231 (IC50 = 57.81 ± 3.49 μg/mL) and HCT116 (IC50 = 58.04 ± 0.96 μg/mL) while MP-EA showed strongest anti-proliferative activity in HCT116 (IC50 = 64.69 ± 0.72 μg/mL). The anticancer potential of MP-HX and MP-EA were also demonstrated by their ability to induce caspase-dependent apoptotic cell death in all of the cancer cell lines tested. Cell cycle analysis suggested that both the MP-HX and MP-EA extracts were able to disrupt the cell cycle in most of the cancer cell lines.

MP-HX and MP-EA extracts demonstrated notable antioxidant, anti-proliferative, apoptosis induction and cancer cell cycle inhibition activities. These findings reflect the promising potentials of MP to be a source of novel phytochemical(s) with health promoting benefits that are also valuable for nutraceutical industry and cancer therapy.


 April 2017


Design, synthesis, and identification of a novel napthalamide-isoselenocyanate compound NISC-6 as a dual Topoisomerase-IIα and Akt pathway inhibitor, and evaluation of its anti-melanoma activity   
Original Research Article
European Journal of Medicinal Chemistry, Available online 21 April 2017, Pages
Deepkamal N. Karelia, Ugir Hossain Sk, Parvesh Singh, A.S. Prakasha Gowda, Manoj K. Pandey, Srinivasa R. Ramisetti, Shantu Amin, Arun K. Sharma

Graphical abstract 



 March 2017


Efficient and reproducible in vitro regeneration of Solanum lycopersicum and assessment genetic uniformity using flow cytometry and SPAR methods   
Original Research Article
Saudi Journal of Biological Sciences, Available online 16 March 2017, Pages
Mohammad Faisal, Abdulrahman A. Alatar, Eslam M. Abdel-Salam, Tomas Canto, Quaiser Saquib, Saad B. Javed, Mohamed A. El-Sheikh, Abdulaziz A. Al-Khedhairy
High resolution UHPLC-MS characterization and isolation of main compounds from the antioxidant medicinal plant Parastrephia lucida (Meyen)   
Original Research Article
Saudi Pharmaceutical Journal, Available online 14 March 2017, Pages



Carlos Echiburu-Chau, Leyla Pastén, Claudio Parra, Jorge Bórquez, Andrei Mocan, Mario J. Simirgiotis

Graphical abstract


Propagation of Brazilian Zika virus strains in static and suspension cultures using Vero and BHK cells   
Original Research Article
Vaccine, Available online 23 March 2017, Pages
Alexander Nikolay, Leda R. Castilho, Udo Reichl, Yvonne Genzel
Involvement of vimentin in neurite outgrowth damage induced by fipronil in SH-SY5Y cells   
Biochemical and Biophysical Research Communications, Available online 18 March 2017, Pages
Theetat Ruangjaroon, Daranee Chokchaichamnankit, Chantragan Srisomsap, Jisnuson Svasti, N. Monique Paricharttanakul


 February 2017


Pharmacological inhibition of DNA methyltransferase 1 promotes neuronal differentiation from rodent and human nasal olfactory stem/progenitor cell cultures

International Journal of Developmental Neuroscience, Available online 1 February 2017, Pages

I. Franco, L. Ortiz-López, B. Roque-Ramírez, G.B. Ramírez-Rodríguez, M. Lamas


Antioxidative and neuroprotective activities of peanut sprout extracts against oxidative stress in SK-N-SH cells

Asian Pacific Journal of Tropical Biomedicine

Pranee Lertkaeo, Apinun Limmongkon, Metawee Srikummool, Tantip Boonsong, Wisa Supanpaiboon, Damratsamon Surangkul

To evaluate the protective effect of peanut sprout extract (PSE) against paraquat (PQ) induced SK-N-SH cells.

Three groups of cells were used in the experiment, together with a fourth, control group. One group was treated with PQ, the second group was treated with PSE, and the third group was pre-treated with PSE. The control group was untreated. Cell viability and toxicity were detected by MTT assay, cellular reactive oxygen species (ROS) was detected by Muse Cell Analyzer, quantitative RT-PCR was applied to investigate the expression of SIRT1 and α-synuclein genes, and Aβ42 was detected by western blot.

The 50% effective concentration of PQ was 0.75 mmol/L. PSE had no significant cytotoxicity at a concentration of 1.5 mg/mL. In the group of cells pre-treated with PSE, cell death was significantly inhibited. In the PQ treated group, PQ was increased in the intracellular ROS in the cells. Intracellular ROS was significantly decreased in the cells treated with PSE and also those pre-treated with PSE. PSE significantly downregulated the expression of SIRT1 and α-syn genes, and it was found that PQ significantly increased β-amyloid 42 levels whereas this action was inhibited by PSE.

PSE has neuroprotective activities against oxidative stress in SK-N-SH cells induced by PQ, suggesting that PSE is a highly promising agent in the prevention of neurodegenerative diseases such as Alzheimer's disease and Parkinson's disease

Diosmin-induced senescence, apoptosis and autophagy in breast cancer cells of different p53 status and ERK activity

Toxicology Letters

Anna Lewinska, Jagoda Adamczyk-Grochala, Ewa Kwasniewicz, Anna Deregowska, Maciej Wnuk

Relatively low bioavailability of plant-derived nutraceuticals with anticancer properties may limit their usefulness for prevention and therapy of cancer. In the present study, we have screened for nutraceuticals (n = 30) that would act at low micromolar range against phenotypically distinct breast cancer cell lines, namely MCF-7 (ER+, PR+/−, HER2−), MDA-MB-231 (ER−, PR−, HER2−) and SK-BR-3 (ER−, PR−, HER2+), and diosmin, a citrus fruit flavonoid belonging to a flavone subclass, was selected. MCF-7 cell line was found to be the most sensitive to diosmin treatment. Diosmin caused G2/M cell cycle arrest, elevation in p53, p21 and p27 levels and stress-induced premature senescence when used at lower concentrations (5 and 10 μM). Diosmin (20 μM) also promoted apoptosis that was not observed in normal human mammary epithelial cells (HMEC). Diosmin stimulated oxidative and nitrosative stress, DNA damage and changes in global DNA methylation patterns. The status of p53 (wild type versus mutant) and the levels of phosphorylated ERK1/2 in a steady state, and diosmin-induced autophagy may reflect diverse response to diosmin treatment in MCF-7, MDA-MB-231 and SK-BR-3 cells, which in turn results in different cell fates. Taken together, diosmin that acts at low micromolar range against breast cancer cells may be considered as a promising candidate for anticancer therapy.


Free Radical Biology and Medicine

Ilaria Crivellari, Claudia Sticozzi, Giuseppe Belmonte, Ximena M. Muresan, Franco Cervellati, Alessandra Pecorelli, Carlotta Cavicchio, Emanuela Maioli, Christos C. Zouboulis, Mascia Benedusi, Carlo Cervellati, Giuseppe Valacchi

For its critical location, the skin represents the major interface between the body and the environment, therefore is one of the major biological barriers against the outdoor environmental stressors. Among the several oxidative environmental stressors, cigarette smoke (CS) has been associated with the development and worsening of many skin pathologies such as acne, dermatitis, delayed wound healing, aging and skin cancer. In our previous work we have demonstrated that CS is able to affect genes involved in skin cholesterol trafficking, among which SRB1, a receptor involved in the uptake of cholesterol from HDL, seems to be very susceptible to the oxidative stress induced by CS.

In the present work we wanted to investigate the presence of SRB1 in human sebocytes and whether CS can affect cholesterol cellular uptake via the redox modulation of SRB1.

By using a co-culture system of keratinocytes/sebocytes, we found that CS exposure induced a SRB1 protein loss without affecting sebocytes viability. The decrease of SRB1 levels was a consequence of SRB1/HNE adducts formation that leads to SRB1 ubiquitination and degradation. Moreover, the CS-induced loss of SRB1 induced an alteration of sebocytes lipid content, also demonstrated by cholesterol quantification in SRB1 siRNA experiments.

In conclusion, exposure to CS, induced SRB1 post-translational modifications in sebocytes and this might affect sebocytes/skin functionality.

Erythro-Austrobailignan-6 down-regulates HER2/EGFR/integrinβ3 expression via p38 activation in breast cancer


Jae-hyun Han, Ha Jin Jeong, Han Na Lee, Yun Ju Kwon, Heung-Mook Shin, Yura Choi, Seongmi Lee, Seung Tack Oh, Dong-il Kim, Songhee Jeon

Despite the benefits from different options of therapy for breast cancer, resistance of the disease to these therapies is rising and a novel agent is needed. Erythro-austrobailignan-6 (EA6) exhibits anti-cancer activity. However, the detailed anti-tumor mechanisms by which EA6 inhibits 4T-1 and MCF-7 cell growth have not been well studied.

In this study, we investigated the anti-proliferative and anti-tumor properties of EA6 on breast carcinoma and its accompanying mechanisms.

The cytotoxic and apoptotic effect of EA6 were measured in breast cancer cell lines of 4T-1 and MCF-7. The role of EA6 on cell proliferation and migration was examined by immunoblotting. The anti-tumor activity of EA6 was assessed in mice inoculated with 4T-1 breast cancer cells.

EA6 increased the number of Annexin V-positive apoptotic bodies and cleaved form of caspase-3 in a dose-dependent manner and phosphorylated JNK and p38 in both cells. Moreover, EA6 down-regulated cell cycle dependent proteins of CDK-4 and cyclin D1, and increased G0/G1 population in both cells. EA6-induced apoptosis is mediated by p38 MAPK and caspase-3 activation in both cells. EA6 significantly reduced HER2/EGFR/integrin β3 expression and Src phosphorylation, which was dependent on p38 MAPK activation in 4T-1 and MCF-7 cells. Furthermore, we confirmed the down-regulation of topoisomerases by EA6 treatment, but the overall effects of EA6 on topoisomerase isotype were cell type specific. Finally, EA6 (20 mg/kg/day) significantly reduced mammary tumor volume in 4T-1 bearing mice by down-regulating HER2/EGFR/integrin β3 expression in tumor tissues.

Our results offer a novel insight into the mechanism of EA6-induced apoptosis in breast cancer cells. We propose that EA6 treatment resulted in the activation of p38 MAPK and caspase-3, which eventually participated in regulating apoptosis in 4T-1 and MCF-7 cells.