GC Analysis of a 38-Component FAME Mix on SP™-2560, 200 m Column

GC Analysis of a 38-Component FAME Mix on SP™-2560, 200 m Column

Conditions

column SP-2560, 200 m x 0.25 mm I.D., 0.20 µm (29688-U)
oven 100 C (13 min), 4 C/min to 240 C (30 min)
carrier gas hydrogen, 27 cm/sec
sample Supelco 37-Component FAME Mix (47885-U) + C22:5n3 FAME
injection 1.0 µL, 50:1 split
liner 4 mm I.D., split type, cup design
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note Identification and quantitation of individual fatty acid isomers in complex mixtures of isomers is desirable for both food and clinical scientists. This application requires the fatty acids to be converted to fatty acid methyl esters (FAMEs), followed by analysis using a long, efficient GC column with a stationary phase highly selective for polarizable analytes (those with double and/or triple C-C bonds). A 200 m SP-2560 is such a column.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Agriculture
Clinical
Food and Beverages
Legal Information SP is a trademark of Sigma-Aldrich Co. LLC
suitability application for GC

Materials

     
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