GC Analysis of Amphetamines in Urine on a 100%Methyl Column after SPME using 100 μm PDMS Fiber

GC Analysis of Amphetamines in Urine on a 100%Methyl Column after SPME using 100 μm PDMS Fiber

Conditions

sample/matrix 1 mL urine + 0.7g potassium carbonate in 20 mL headspace vial, equilibrated at 80C, 30 min
derivatization methyl bis-trifluoroacetamide (headspace, 0.5 min, ambient)
desorption process 1 min, 270 °C
extraction headspace, 3-5 min, 80 °C
SPME fiber polydimethylsiloxane, 100 µm (57300-U)
column 100% methyl polysiloxane, 12.5 m x 0.2 mm I.D., 0.33 µm (Supelco equivalent, Equity-1, 28041-U)
oven 60 °C (1 min) to 140 °C (4 min) at 30 °C/min, then to 276 C at 20 °C/min, 4 min
inj. temp. splitless (closed 1 min), 270 °C
detector MS, full scan

Description

Categories Analytical Chromatography, SPME Applications, GC Applications, Drugs & Pharmaceuticals, Drugs of Abuse, Amines, Pharmaceuticals
Featured Industry Clinical
Forensics and Toxicology
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Materials

     
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