GC Analysis of Chemical Signals from Spruce Seedlings (Healthy vs. Wounded) on a 5%Phenyl Column after SPME using 100 μm PDMS Fiber

GC Analysis of Chemical Signals from Spruce Seedlings (Healthy vs. Wounded) on a 5%Phenyl Column after SPME using 100 μm PDMS Fiber

Conditions

sample/matrix headspace surrounding Picea abies seedlings
desorption process 225 °C for 30 sec
extraction headspace, 60 min.
SPME fiber 100 µm polydimethylsiloxane (57300-U)
column 5% phenyl polysiloxane, 30 m x 0.25 mm I.D., 0.25 µm (Supelco equivalent, Equity-5, 28089-U)
oven 40 °C (4 min), 4 °C/min to 200 °C (16 min)
carrier gas helium, 10 psi
detector EID, 70ev

Description

Categories Analytical Chromatography, SPME Applications, GC Applications, Agriculture, Volatiles
Featured Industry Agriculture
suitability application for SPME, application for GC

Materials

     
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