GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method Ce 1h-05

GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method Ce 1h-05

Conditions

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056)
oven 180 C
carrier gas helium, 1 ml/min
sample cis/trans FAME Column Performance Mix (40495-U)
injection 1 µL, 100:1 split
inj. temp. 250 C
detector FID, 250 C

Description

Analysis Note Complex mixtures of cis/trans FAME isomers are difficult to separate. Baseline resolution may not be possible for some samples due to variation in GC instrument designs and column efficiencies. As a result, AOAC Method Ce 1h-05 recommends monitoring changes in column performance with a known FAME mixture closely matching the range of fatty acids under investigation.

The resolution factor for Peak 1 and Peak 2 of this mix (40495-U) is a good indicator of colum performance. Using one of the resolution equations listed on the Data Sheet, the resolution factor can be calculated. Resolution factors approaching 1.5 (baseline resolution) are desired.

For additional information, request Data Sheet T708019.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Agriculture
Clinical
Food and Beverages
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suitability application for GC

Materials

     
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