GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method 2012.13

GC Analysis of cis/trans FAME Column Performance Mix on SP™-2560 per AOAC Method 2012.13

Conditions

column SP-2560, 100 m x 0.25 mm I.D., 0.20 µm (24056)
oven 60 °C (1 min), 15 °C/min to 165 °C (1 min), 2 °C/min to 225 °C (20 min)
carrier gas helium, 0.8 mL/min
sample cis/trans FAME Column Performance Mix (40495-U)
injection 1 µL, 10:1 split
liner 4 mm I.D., split/splitless type, wool packed single taper FocusLiner™ design
inj. temp. 250 °C
detector FID, 250 °C

Description

Analysis Note Prior to analysis of samples, AOAC Method 2012.13 requires that system resolution be verified to ensure sufficient separation of the C18:1 cis/trans isomers. This is done by injecting a cis/trans FAME column performance mix (40495-U), a qualitative mix that contains a mixture of C18:1 trans and C18:1 cis isomers. Resolution is calculated between two groups of FAME isomers. Group 1 = C18:1Δ13t and C18:1Δ14t, whereas group 2 = C18:1Δ9c (oleic acid) and C18:1Δ10c. A resolution value of >1.0 is considered acceptable. As shown, the resolution value calculated using the SP-2560 column is R=1.12.
Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Agriculture
Clinical
Food and Beverages
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suitability application for GC

Materials

     
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