GC Analysis of FAMEs in Cookies on SLB®-IL111 after SPE Fractionation using Discovery® Ag-Ion

GC Analysis of FAMEs in Cookies on SLB®-IL111 after SPE Fractionation using Discovery® Ag-Ion

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 1 g of commercially purchased cookies was ground and subjected to acid digestion and alkali hydrolysis followed by methylation as described in AOCS Official Method Ce 1k-09
SPE tube/cartridge Discovery Ag-Ion SPE tubes, 750 mg/6 mL (54225-U)
condition 4 mL of acetone; allow solvent to gravity drip completely through tube; discard eluant; 4 mL of hexane; allow solvent to gravity drip completely through tube; discard eluant
sample addition 1 mL of extract; discard any eluant that drips through tube
elution (Fraction 1) 6 mL of hexane:acetone (96:4); collect eluant in a fresh container with slight vacuum; ((Fraction 2) 4 mL of hexane: acetone (90:10); collect eluant in a fresh container with slight vacuum; (Fraction 3) 4 mL of 100% acetone; collect eluant in a fresh container with slight vacuum)
eluate post-treatment evaporate each fraction at room temperature using nitrogen; reconstitute each fraction to 1 mL of hexane
column SLB-IL111, 100 m x 0.25 mm I.D., 0.20 µm (29647-U)
oven 168 °C
carrier gas 1 µL, 10:1 split
injection 1 µL, 10:1 split
liner 4 mm I.D., split type, single taper wool packed FocusLiner™ design
inj. temp. 250 °C
detector FID, 250 °C

Description

Analysis Note A sample of commercially purchased cookies was ground, and fatty acids were extracted, methylated, fractionated, and analyzed with GC-FID using the SLB-IL111. The nutritional label for the cookies included partially hydrogenated cottonseed and soybean oils as ingredients. Based on this information, the fatty acid profile obtained for this sample was as expected; with cottonseed oil accounting for the small amount of C14:0 FAME, and the soybean oil as the source of the small amount of C22:0 FAME detected. Both oils contributed to the higher levels of the C16:0, C18:0 and C18:1 FAMEs. Note the presence of the C18:1 trans isomers in fraction 1. These are the result of the partial hydrogenation of these oils.
Categories Analytical Chromatography, SPE Applications, GC Applications, FAMEs (Fatty Acid Methyl Esters), Fatty Acid Methyl Esters (FAMEs)
Featured Industry Food and Beverages
Legal Information FocusLiner is a trademark of SGE Analytical Science Pty Ltd., Discovery is a registered trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
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suitability application for GC, application for SPE

Materials

     
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