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GC Analysis of FAMEs in Human Plasma on SLB®-5ms

GC Analysis of FAMEs in Human Plasma on SLB®-5ms

Conditions

sample/matrix 1.5 mL human plasma
extraction Lipid fraction obtained according to the Folch method
column SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U)
oven 50 C, 3 C/min to 280 C
carrier gas helium, 35 cm/sec
injection 1 µL, 10:1 split
liner 4 mm I.D., split/splitless type, wool packed straight FocusLiner(TM) design
inj. temp. 250 C
detector MSD, 220 C, m/z = 35-550
MSD interface 250 C

Description

Categories Analytical Chromatography, GC Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Clinical
Legal Information FocusLiner is a trademark of SGE Analytical Science Pty Ltd., SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes eluate post-treatment: Approximately 100 μL of the lipid fraction transferred to a Pyrex tube, dried under nitrogen, and subsequently transesterified with 1 mL of a sodium methoxide/methanol mixture (0.5 %, w/v) at 100 °C for 15 min; after cooling, 1 mL of boron trifluoride-methanol added, and the solution heated at 100 °C for 15 min; after cooling, 1.0 mL of n-hexane, 1.0 mL of a sodium chloride saturated solution in distilled water, and anhydrous sodium sulfate were added to the mixture, agitated manually for 1 min, and centrifuged at 4000 rpm for 2 min; approximately 1.0 mL of the upper n-hexane layer transferred to a 2.0 mL autosampler vial and stored at 4 °C until analysis
suitability application for GC

Materials

     
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