GC Analysis of FAMEs in Popcorn Oil on SP™-2560 (75 m x 0.18 mm I.D., 0.14 μm) after SPE Fractionation using Discovery® Ag-Ion, Fast GC Analysis

GC Analysis of FAMEs in Popcorn Oil on SP™-2560 (75 m x 0.18 mm I.D., 0.14 μm) after SPE Fractionation using Discovery® Ag-Ion, Fast GC Analysis

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 1 g of popcorn oil FAME's in Hexane
SPE tube/cartridge Discovery Ag-Ion, 750 mg/6 mL (54225-U)
condition 4 mL acetone and 4 mL hexane
sample addition 1 mL of the hexane FAME extract
elution 6 mL hexane:acetone (96:4, v/v) followed by 4 mL hexane:acetone (90:10, v/v)
column SP-2560, 75 m x 0.18mm I.D., 0.14 µm (23348-U)
oven 180 Cisothermal
carrier gas hydrogen, 40 cm/sec. at 180 C
injection (0.5 µL, 100:1 split)
liner 0.5 µL, 100:1 split
inj. temp. 220 C
detector FID, 220 C

Description

Analysis Note Discovery Ag-Ion operates by argentation chromatography which is a powerful analytical and sample prep technique for the separation or fractionation of cis and trans geometric and positional fatty acid (FA), fatty acid methyl ester (FAME), diacylglycerol (DAG) and triacylglycerol (TAG) isomers. Discovery Ag-Ion SPE is shown in this application to separate FAMEs from an edible oil sample.
Categories Analytical Chromatography, SPE Applications, GC Applications, FAMEs (Fatty Acid Methyl Esters), Food Industry Applications, Fatty Acid Methyl Esters (FAMEs)
Featured Industry Agriculture
Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC, SP is a trademark of Sigma-Aldrich Co. LLC
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Preparation Note Sample pretreatment: A 1 g sample of popcorn oil was scraped from the inside of a popped microwave oven popcorn bag and mixed with 8 mL deionized water (DI) and 4 mL petroleum ether, centrifuged to remove particulates. The ether layer (upper layer) was collected. This petroleum ether extraction step was repeated four times. The upper ether layers were combined, evaporated, and reconstituted in 16 mL toluene. A 1 mL aliquot was transferred to conical reaction vial and 2 mL 7% boron triflluoride in methanol were added. The reaction was incubated at 80 °C for 15 minutes using a heating block, then cooled to room temperature. 1 mL DI water was added and FAMEs were extracted twice using 1 mL hexane each time. The upper hexane layers were combined into a fresh vial, evaporated, and reconstituted in 5 mL hexane and 50 mg anhydrous sodium sulfate.
suitability application for GC, application for SPE

Materials

     
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