GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean™ ENVI-Carb™ II/PSA

GC Analysis of Organophosphorus Pesticides (OP-Pest) in Cabbage, Onion, and Mushrooms on SLB-5ms after SPE using Supelclean™ ENVI-Carb™ II/PSA

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix 10 g homogenized food sample (green onions, apples, Napa cabbage, button mushrooms)
SPE tube/cartridge Supelclean ENVI-Carb II/PSA SPE Tube (54067-U)
condition 5 mL acetone:toluene (65:35)
sample addition 1 mL sample extract
elution 10 mL of acetone:toluene (65:35)
eluate post-treatment Evaporate fraction to 0.5 mL then adjust volume to 1 mL with ethyl acetate for GC-NPD analysis
column SLB-5ms, 30 m x 0.25 mm I.D., 0.25 µm (28471-U)
oven 110 C (2 min), 2.5 C/min to 205 C, 10 C/min to 310 C (10 min)
carrier gas helium, 0.9 ml/min constant flow
injection 2 µL, splitless
liner 4 mm I.D. dual tapered
inj. temp. 250 C
detector NPD, 310 C

Description

Analysis Note Organophosphorous pesticide use in agriculture is widespread due to the fact that they are more amenable to environmental degradation in comparison to organochlorine or organonitrogen compounds. There are a large number of pesticides in the organophosphorous group, and because of their potential health effects, they are of particular concern on produce imported from areas in which they are commonly used. This chromatogram illustrates the separation of 63 of these pesticides by GC on the SLB-5ms using nitrogen phosphorus detection (NPD).
Categories Analytical Chromatography, SPE Applications, GC Applications, Pesticides
Featured Industry Agriculture
Food and Beverages
Legal Information ENVI-Carb is a trademark of Sigma-Aldrich Co. LLC, Supelclean is a trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
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Septa and Specialized Hand Tools
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Preparation Note Sample pretreatment: To a 10 g sample of homogenized food (green onions, apples, Napa cabbage, button mushrooms) was added 10 mL acetonitrile and 4 g magnesium sulfate (that had been heated at 550 °C) and 1 g sodium chloride. After centrifugation, the supernatant was removed and mixed with 1 g magnesium sulfate and centrifuged. 5 mL of the supernatant was removed and evaporated to 1 mL for SPE loading.
suitability application for GC, application for SPE

Analyte

  • Acephate
  • Azinphos-ethyl
  • Azinphos-methyl
  • Bromophos-ethyl
  • Cadusafos
  • Chloropyrifos
  • Chloropyrifos-methyl
  • CIPC
  • Coumaphos
  • Demeton-ethyl
  • Diazinon
  • Dibromfos
  • Dichlofenthion
  • Dichlorvos
  • Dicrotophos
  • Dimethoate
  • Disulfoton
  • Dyfonate
  • Edinphos
  • EPN
  • Ethion
  • Ethoprophos
  • Famphur
  • Fenitrothion
  • Fensulfothion
  • Fenthion
  • Formothion
  • Fosthiazate
  • Fosthiazate
  • Isazofos
  • Isocarbophos
  • Isophenphos
  • Isophenphos-methyl
  • Kitazine
  • lodophenphos
  • Malathion
  • Methamidophos
  • Methidathion
  • Methyl parathion
  • Monocrotophos
  • Omethoate
  • Paraoxon-ethyl
  • Parathion
  • Phenthoate
  • Phorate
  • Phosalone
  • Phosdrin
  • Phosfolan
  • Phosmet
  • Phosphamidon
  • Pirmiphos-ethyl
  • Pirmiphos-methyl
  • Profenofos
  • Propetamphos
  • Pyridafenthion (pyridaphenthion)
  • Quinalphos
  • Ronnel
  • Sulfotep
  • Temephos
  • Terbufos
  • Thiometon
  • Trichloronate
  • Trichlorphan

Materials

     
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