GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis

GC Analysis of Pesticides in Avocado on SLB®-5ms (20 m x 0.18 mm I.D., 0.36 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep+, Fast GC Analysis

Conditions

sample preparation Dispersive (QuEChERS)
sample/matrix Avocado spiked with pesticides at 20 ng/g
extraction tube Supel QuE Acetate extraction tube (55234-U)
extraction process add 25 mL of acetonitrile; shake for 1 minute; add contents of Supel QuE acetate extraction tube; shake immediately for 1 minute; centrifuge at 3200 rpm for 5 minutes
clean-up process transfer 3 mL of the acetonitrile layer into a Supel Que Z-Sep+ cleanup tube; shake for 1 minute; centrifuge at 3400 rpm for 3 minutes; draw off supernatant for LVI-GCMS analysis
clean-up tube Supel Que Z-Sep+ cleanup tube (55296-U)
column SLB-5ms, 20 m x 0.18 mm I.D., 0.36 µm (28576-U)
oven 70 C (2 min), 15 C/min to 325 C (6 min)
carrier gas helium, 1 ml/min, constant
sample pesticides spiked at 20 ng/g
injection PTV solvent vent; 10 µL, injection dispense speed 15000 µL/min
liner 4 mm I.D. FocusLiner with taper
inj. temp. 60 C (0.28 min), 600 C/min to 325 C (5 min)
detector MSD
MSD interface 330 C

Description

Analysis Note About this analysis: This application demonstrates the use of dispersive SPE for the extraction of pesticides from Avocado. Cleanup is achieved using Z-Sep + for fat removal. FInal analysis of the extracts was done by LVI-GC/MS SIM on an SLB-5ms column.
Categories Analytical Chromatography, SPE Applications, GC Applications, Pesticides
Featured Industry Agriculture
Food and Beverages
Legal Information SLB is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC, application for SPE

Analyte

  • 4,4′-DDT
  • Chloropyrifos
  • Coumaphos
  • Cyflurthrin
  • Cypermethrin
  • Deltamethrin
  • Dimethoate
  • Endosulfan I
  • Heptachlor
  • Hexachlorobenzene
  • Malathion
  • Methoxychlor
  • Metolachlor
  • Quintozene
  • Simazine
  • Trifluralin
  • α-BHC
  • γ-BHC

Materials

     
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