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GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Salmon on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Salmon on SPB®-608 (20 m x 0.18 mm I.D., 0.18 μm) after QuEChERS Cleanup using Supel™ QuE Z-Sep, Fast GC Analysis

Conditions

sample preparation Dispersive (QuEChERS)
sample/matrix raw salmon spiked with PAHs at 100 ng/g
extraction tube Supel(TM) QuE Non-buffered tube, magnesium sulfate/sodium chloride (55294-U)
extraction process add 25 mL acetonitrile and the contents of the Supel(TM) QuE Non-buffered tube (55294-U); shake for 5 minutes; centrifuge for 5 minutes at 3200 rpm
clean-up process transfer 3 mL of acetonitrile layer, Supel QuE Z-Sep (500 mg) and MgSO4 (450 mg) into an extraction tube; (shake 1 minute; centrifuge for 3 minutes at 3400 rpm; remove 1 mL supernatant for GC-MS analysis)
clean-up tube Supel(TM) QuE Z-Sep (500 mg, part no. 55299-U), magnesium sulfate (450 mg, part no. 63135) (55299-U, 63135)
column SPB-608, 20 m x 0.18 mm I.D. x 0.18 µm
oven 60 C (1 min), 25 C/min to 275 C, 10 C/min to 300 C (13 min)
carrier gas helium, 1.5 ml/min, constant
injection 1 µL, splitless (1 min)
liner 4 mm I.D. FocusLiner(TM) with taper
inj. temp. 265 C
detector MS, SIM
MSD interface 300 C

Description

Analysis Note This application demonstrates the use of dispersive SPE or QuEChERS for the extraction and cleanup of raw salmon for GC-MS analysis of polynuclear aromatic hydrocarbons (PAHs). Supel QuE Z-Sep sorbent was used in the cleanup step and found to reduce background and yield recoveries >80% for a majority of the PAHs tested., Sample pretreatment: Place 5 g ground sample into 50 mL centrifuge tube. Spike with 100 ng/g target PAHs and internal standards. Let sit 30 min. Add 5 mL water and homogenize.
Application Extraction solvent: acetonitrile, 25 mL
Clean-up volume: 3 mL
Clearn-up tube: Supel QuE Z-Sep (500 mg, part no. 55299-U, magnesium sulfate (450 mg, part no. 63135)
Categories Analytical Chromatography, SPE Applications, GC Applications, Hydrocarbons, PAHs
Featured Industry Food and Beverages
General description Stenerson, K.; Ye, M.; Halpenny, M., New Analytical Tools for the Determination of Persistent Organic Pollutants (POPs) in Fatty Food and Beverage Matrices Using QuEChERS Extraction/Cleanup and Gas Chromatography (GC) Analysis. Pittcon 2013. Sigma-Aldrich/Supelco publication T413034
Legal Information SPB is a registered trademark of Sigma-Aldrich Co. LLC, Supel is a trademark of Sigma-Aldrich Co. LLC, FocusLiner is a trademark of SGE Analytical Science Pty Ltd.
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for GC, application for SPE

Analyte

  • 1-Methylnaphthalene
  • 1-Methylphenanthrene
  • 2,3,5-Trimethylnaphthalene
  • 2,6-Dimethylnaphthalene
  • 2-Methylnaphthalene
  • Acenaphthene
  • Acenaphthylene
  • Anthracene
  • Benzo[a]anthracene
  • Benzo[a]pyrene
  • Benzo[b]fluoranthene
  • Benzo[e]pyrene
  • Benzo[g,h,i]perylene
  • Benzo[j]fluoranthene
  • Benzo[k]fluoranthene
  • Biphenyl
  • Chrysene
  • Dibenzo[a,h]anthracene
  • Dibenzothiophene
  • Fluoranthene
  • Fluoranthene-d10
  • Fluorene
  • Indeno[1,2,3-cd]pyrene
  • Naphthalene
  • Naphthalene-d8
  • Perylene
  • Perylene-d12
  • Phenanthrene
  • Pyrene
  • Retene
  • Triphenylene

Materials

     
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