GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Soybean Oil on SLB®-35ms after SPE using Supelclean™ EZ-POP NP

GC Analysis of Polynuclear Aromatic Hydrocarbons (PAHs) in Soybean Oil on SLB®-35ms after SPE using Supelclean™ EZ-POP NP

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix soybean oil spiked with PAHs at 10 ng/g
SPE tube/cartridge Supelclean EZ-POP NP, 2.5 g/12 mL (54341-U)
condition 10 mL acetone, dry under 10-15" Hg for 10 minutes
sample addition 0.5 mL weighed accurately onto cartridge
elution 15 mL acetonitrile
eluate post-treatment concentrate to final volume of 0.5 mL at 40 °C under nitrogen
column SLB-35ms, 30 m x 0.25 mm I.D., 0.25 µm (29804-U)
oven 60 °C (1 min), 20 °C/min to 340 °C (10 min)
carrier gas helium, 1 mL/min constant flow
liner 2 mm I.D. FocusLiner with taper
inj. temp. 300 °C
detector MSD-SIM (MS source at 250 °C, quads at 200C)
MSD interface 330 °C
scan range SIM

Description

Analysis Note PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying. In this application, EZ-POP NP SPE is used to extract PAHs from two popular edible oils, canola and soybean. The 16 PAHs designated in many US EPA methods were analyzed by GC/MS after extraction from spiked samples of oil.
Categories Analytical Chromatography, SPE Applications, GC Applications, Hydrocarbons, PAHs
Featured Industry Agriculture
Food and Beverages
Legal Information Supelclean is a trademark of Sigma-Aldrich Co. LLC, SLB is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for GC, application for SPE

Analyte

  • Acenaphthene
  • Acenaphthylene
  • Anthracene
  • Benzo[a]anthracene
  • Benzo[a]pyrene
  • Benzo[b]chrysene
  • Benzo[b]fluoranthene
  • Benzo[g,h,i]perylene
  • Benzo[k]fluoranthene
  • Chrysene
  • Dibenzo[a,h]anthracene
  • Fluoranthene
  • Fluorene
  • Indeno[1,2,3-cd]pyrene
  • Naphthalene
  • Phenanthrene
  • Pyrene

Materials

     
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