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GC Analysis of Stored Apples on 5% Phenyl Column after SPME using 100 μm PDMS Fiber

GC Analysis of Stored Apples on 5% Phenyl Column after SPME using 100 μm PDMS Fiber

Conditions

sample/matrix 300-450g intact fruit in a 3 liter flask at ambient temp, exposed to an air flow of 25-30 ml/min
desorption process 250C for 90 sec
SPME fiber polydimethylsiloxane, 100 µm film (57310-U)
column 5% phenyl polysiloxane, 25m x 0.25 mm I.D., 0.34 µm film (Supelco equivalent, SLB-5ms, 28473-U)
oven 40 C (1.5 min) to 250 C at 50 C/min, hold 1 min
carrier gas helium, 1.5 ml/min (44 cm/min)
sample Apples
inj. temp. 250 C
detector mass spectrometer, m/z = 40-300 (40 spectra/sec)

Description

Analysis Note SPME can reduce sampling time and is complementary to rapid separation/detection techniques such as time compressed gas chromatography (TCGC) and time-of-flight mass spectrometry (TOF-MS), to permit analysis of dozens of compounds in a few minutes. Investigators have used SPME to monitor flavor volatiles in apple, strawberry, tomato, and many other fruits and vegetables. In this application, 65 μm PDMS/divinylbenzene (DVB) SPME fibers were used to capture volatiles emanating from Mutsu apples stored for 3-4 months at 0 °C.
Categories Analytical Chromatography, SPME Applications, GC Applications, Foods, Flavors & Fragrances, Volatiles, Agriculture
Featured Industry Agriculture
Food and Beverages
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • 2-Methylbutanol
  • 2-Methylbutyl acetate
  • 2-Methylpropyl-2-methylbutanoate
  • 2-Pentenylhexanoate
  • 3-Methylbutylhexanoate
  • Butyl acetate
  • Butyl hexanoate
  • Butyl-2-methylbutanoate
  • Hexanol
  • Hexyl acetate
  • Hexyl butanoate
  • Hexyl hexanoate
  • Hexyl-2-methylbutanoate
  • Methylpropylbutanoate
  • Pentyl butanoate
  • Pentyl hexanoate
  • p-Methyoxyalylbenzene
  • α-Farnesene

Materials

     
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