GC Analysis of Tricyclic Antidepressants in Urine on a 100%Methyl Column after SPME using 100 μm PDMS Fiber

GC Analysis of Tricyclic Antidepressants in Urine on a 100%Methyl Column after SPME using 100 μm PDMS Fiber

Conditions

sample/matrix 1 mL urine (1 mug each analyte + 50 µl 5 M NaOH) in 7.5 mL vial
desorption process 3 min, 280 C
extraction headspace, 15 min, 100 C (sample incubated 30 min)
SPME fiber polydimethylsiloxane, 100 µm (57300-U)
column 100% methyl polysiloxane, 30 m x 0.32 mm I.D., 0.25 µm (Supelco equivalent, Equity-1, 28055-U)
oven 100 C to 300 C at 20 C/min
carrier gas helium, 30 cm/sec
inj. temp. splitless (splitter opened after 1 min), 280 C
detector FID, 280 C

Description

Categories Analytical Chromatography, SPME Applications, GC Applications, Drugs & Pharmaceuticals, Pharmaceuticals
Featured Industry Clinical
Forensics and Toxicology
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Materials

     
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