Attention:

Certain features of Sigma-Aldrich.com will be down for maintenance the evening of Friday August 18th starting at 8:00 pm CDT until Saturday August 19th at 12:01 pm CDT.   Please note that you still have telephone and email access to our local offices. We apologize for any inconvenience.

GC Analysis of Volatiles in Green Arabica Coffee on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Volatiles in Green Arabica Coffee on SUPELCOWAX® 10 after SPME using 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

sample/matrix Arabica coffee beans, green, 2 grams with pre-equilibration of 10 min at 60 C, the entire process under agitation at 500 rpm
desorption process 5 min
extraction headspace, 40 min at 60 C
SPME fiber divinylbenzene/carboxen on polydimethylsiloxane, 50/30 µm (57328-U)
column SUPELCOWAX 10, 30 m x 0.25 mm I.D., 0.25 µm (24079)
oven 40 C (5 min), 4 C/min to 230 C, 50 C/min to 280C (2 min)
carrier gas helium, 34.0 cm/s and 40.3 KPa
injection splitless 5 min, then split ratio 1:20
liner 0.75 mm i.d.
inj. temp. 260 C
detector MS (Shimadzu GCMS-QP2010) ionization energy 1.5 kV; mass range: 40-400 amu; scan mode, scan interval : 0.5 sec
MSD interface 230 C, source temp. 200 C

Description

Categories Analytical Chromatography, SPME Applications, GC Applications, Food & Beverage Analysis, Foods, Flavors & Fragrances, Beverage Testing, Volatiles, Coffee and Tea
Featured Industry Food and Beverages
Legal Information SUPELCOWAX is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • Volatile compounds from Green Arabica coffee

Materials

     
Related Links