GC Analysis of Volatiles in Roasted Coffee Beans on Omegawax® after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

GC Analysis of Volatiles in Roasted Coffee Beans on Omegawax® after SPME using a 50/30 μm DVB/Carboxen/PDMS Fiber

Conditions

sample/matrix 2 g of coffee beans
desorption process 260 C for 5 min
extraction headspace, with a pre-equilibration period of 10 min. at 60 C, and then a fiber exposure of 40 min. at 60 C, agitated in an alternate clockwise-anticlockwise rotation mode at 500 rpm
SPME fiber 50/30 µm divinylbenzene/Carboxen on polydimethylsiloxane on a StableFlex fiber (57329-U)
column Omegawax 250, 30 m x 0.25 mm I.D., 0.25 µm (24136)
oven 40 C (5 min), 4 C/min to 230 C, 50 C/min to 280 C (2 min)
carrier gas helium, 34.8 cm/sec constant
sample Roasted coffee
injection splitless
liner 0.75 mm I.D. SPME/Splitless (2633901)
inj. temp. 260 C
detector MS, m/z 40-400
scan range m/z 40-400

Description

Analysis Note Headspace-SPME followed by GC analysis was used in this application to analyze volatile compounds in roasted coffee. SPME is ideal for analyzing the headspace of solid samples like coffee beans. The Carboxen/DVB/PDMS fiber is the fiber of choice for analyzing the wide range of analytes commonly found in roast coffee beans.The relatively polar Omegawax 250 GC column provided high resolution.
Categories Analytical Chromatography, SPME Applications, GC Applications, Food & Beverage Analysis, Foods, Flavors & Fragrances, Beverage Testing, Volatiles, Coffee and Tea
Featured Industry Food and Beverages
Legal Information Omegawax is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes Complementary products:
Syringes
Vials
Gas Purification and Gas Management
Inlet Liners, Glass Wool, and Specialized Hand Tools
Septa and Specialized Hand Tools
Column Ferrules, Nuts, and Specialized Hand Tools
suitability application for SPME, application for GC

Analyte

  • 1-(2-Furyl)-3-butanone
  • 1-(5-Methyl-2-furyl)-2-propanone
  • 2,3,5-Trimethylpyrazine
  • 2,3-Butanedione
  • 2,3-Dihydro-5-methylfuran
  • 2,3-Dimethylpyrazine
  • 2,3-Hexanedione
  • 2,3-Pentanedione
  • 2,5-Dimethylfuran
  • 2,5-Dimethylpyrazine
  • 2,5-Dimethylpyrrole
  • 2,6-Dimethylpyrazine
  • 2-Acetyl-1-methylpyrrole
  • 2-Acetylfuran
  • 2-Acetylpyrrole
  • 2-Butanone
  • 2-Ethyl-5-methylpyrazine
  • 2-Ethyl-6-methylpyrazine
  • 2-Ethylpyrazine
  • 2-Furfuryl furan
  • 2-Furfuryl-5-methylsulfide
  • 2-Methoxyphenol
  • 2-Methyl-2-cyclopentenone
  • 2-Methylbutanal
  • 2-Methylfuran
  • 2-Methylpyrazine
  • 2-Vinyl-5-methylfuran
  • 3,4-Hexanedione
  • 3-Butenone
  • 3-Ethylpyridine
  • 3-Hexanone
  • 3-Methylbutanal
  • 3-Penten-2-one
  • 4-Heptanone
  • 4-Pyran-4-one
  • 5-Methylfurfural
  • Acetoin
  • Acetol acetate
  • Dimethyl formaldehyde
  • Ethyl acetate
  • Ethyl alcohol
  • Furfural
  • Furfuryl acetate
  • Furfuryl alcohol
  • Furfuryl ether
  • Furfuryl formate
  • Furfuryl pyrrole
  • Hexanal
  • Limonene
  • Linalool
  • Linalyl acetate
  • Methyl acetate
  • Methyl-1H-pyrrole
  • N-Acetyl-4(H)-pyridine
  • N-Methyl-2-formylpyrrole
  • p-Cymene
  • Phenylethyl alcohol
  • Pyrazine
  • Pyridine
  • Pyrrole
  • Pyrrole-2-carboxaldehyde
  • Sabinene
  • α-Terpinene
  • β-Myrcene
  • β-Pinene
  • γ-Butyrolactone
  • γ-Terpinene

Materials

     
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