SFC Analysis of Warfarin™ Enantiomers on Astec® Cellulose DMP, Methanol Gradient

SFC Analysis of Warfarin™ Enantiomers on Astec® Cellulose DMP, Methanol Gradient

Conditions

column Astec Cellulose DMP, 15 cm x 4.6 mm I.D., 5 µm particles (51098AST)
column temp. 35 C
mobile phase [A] carbon dioxide; [B] methanol; Gradient of 5-65% methanol in 3 min., hold for 1 min
flow rate 4 ml/min
pressure 1450 psi (100 bar)
sample warfarin
injection 5 µl
detector UV, 220 nm

Description

Analysis Note SFC data provided by Dr. Christina Kraml, Lotus Separations, LLC, Princeton, NJ.
Instrument: Berger Analytical SFC system equipped with a dual pump (FCM-1200), autosampler (ALS 3100), column oven (TCM-200) and diode-array detector (DAD-4100); from Mettler Toledo.
Categories Analytical Chromatography, SFC Applications, Chiral Applications, Clinical, Forensic & Toxicology, Therapeutic Drug Monitoring
Featured Industry Clinical
Pharmaceutical (small molecule)
Legal Information Warfarin is a trademark of Wisconsin Alumni Research Foundation, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for SFC

Materials

     
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