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High Efficiency Resolution on Ascentis® Express 2.7 μm Versus 3μm Particles

High Efficiency Resolution on Ascentis® Express 2.7 μm Versus 3μm Particles

Conditions

column Ascentis Express C18, 15 cm x 4.6 mm I.D., 2.7 µm particles and C18, 15 cm x 4.6 mm I.D., 3 µm particles (53829-U)
column temp. ambient
mobile phase [A] water; [B] acetontrile; (35:65 or 27.5:72.5, A:B)
flow rate 1.5 ml/min
injection 2 µl
detector UV, 220 nm

Description

Categories Analytical Chromatography, Semivolatiles
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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