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HILIC Analysis of Polar Vitamins on Ascentis® Express OH5

HILIC Analysis of Polar Vitamins on Ascentis® Express OH5


column 35 C
column Ascentis Express OH5 10 cm x 3.0 mm I.D. x 2.7 µm particles (53769-U)
mobile phase 100 mM ammonium formate:Acetonitrile (15:85, v/v), pH adjusted to 5.0 with formic acid
flow rate 0.5 ml/min
pressure 850 psi (58.6 bar)
sample 100 ug/mL in 50:50 methanol:acetonitrile
injection 2 µl
detector UV, 276 nm


Analysis Note This application demonstrates good retention and selectivity for polar vitamins on Ascentis® Express OH5 in HILIC mode.
Categories Analytical Chromatography, Clinical, Pharmaceutical Analysis, Anticonvulsant/antiepiletic/antiseizure, vitamins, antiepileptic/antiseizure, Vitamins and Natural products
Featured Industry Clinical
Vitamins, Nutraceuticals, and Natural Products
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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