HPLC Analysis MAb Variants on Proteomix® SCX-NP5, Fast Analysis

HPLC Analysis MAb Variants on Proteomix® SCX-NP5, Fast Analysis

Conditions

column Proteomix SCX-NP5, 25 cm x 4.6 mm I.D., 5 µm particles (Z777164)
mobile phase [A] 2.4 mM Tris, 1.5 mM imidazole, 11.6 mM piperazine, pH 6.0; [B] A + 0.5 M NaCl, pH 10.5
gradient top (0.8 ml/min): 5% B held for 5 min; to 29.5% B in 35 min; bottom (1.5 ml/min): 5% B held for 2.7 min; to 29.5% B in 18.6 min
flow rate 0.8 ml/min or 1.5 ml/min
sample MAb 321, 5 mg/mL
injection 20 µl
detector UV 280 nm

Description

Analysis Note Due to the low pressure of Proteomix SCX, faster analysis and high resolution can be achieved with a higher flow rate.
Categories Analytical Chromatography, Antibiotics, Antimicrobials
Featured Industry Life Science and Biopharma
Legal Information Proteomix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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