HPLC Analysis of 2-AMP and Piroxicam on Ascentis® Express F5

HPLC Analysis of 2-AMP and Piroxicam on Ascentis® Express F5

Conditions

column Ascentis Express F5, 10 cm x 4.6 mm I.D., 2.7 µm particles (53590-U)
column temp. 35 °C
mobile phase [A] 10 mM ammonium formate, pH=3; [B] acetonitrile; (75:25, A:B)
flow rate 0.8 mL/min
pressure 1635 psi (113 bar)
sample 100 µg/mL Piroxicam and 10 µg/mL 2-AMP in mobile phase
injection 5 µL
detector UV, 230 nm

Description

Categories Analytical Chromatography, Anti-inflammatories, Proteins
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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