HPLC Analysis of 2-Bromo-3-Methylbutyric Acid Enantiomers on Astec® CHIROBIOTIC® R

HPLC Analysis of 2-Bromo-3-Methylbutyric Acid Enantiomers on Astec® CHIROBIOTIC® R

Conditions

column CHIROBIOTIC R, 25 cm x 4.6 mm I.D., 5 µm particles (13024AST)
column temp. 25 °C
mobile phase [A] 20 mM ammonium acetate; [B] methanol; (15:85, A:B)(alternate mobile phase of 0.1% ammonium acetate in methanol gives 3.5 and 4.3 min retention)
flow rate 1 mL/min
sample 2 mg/mL in methanol
injection 5 µL
detector UV, 254 nm

Description

Categories Analytical Chromatography, Chiral Applications, Organic Chemicals, Organic Acids, Chemicals and Industrial Polymers, Other Organic Compound Applications
Featured Industry Chemicals and Industrial Polymers
Legal Information CHIROBIOTIC is a registered trademark of Sigma-Aldrich Co. LLC, Astec is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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