HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease

HPLC Analysis of a Monoclonal Antibody on Zenix® SEC-300 after Partial Proteolysis with IdeS Protease

Conditions

column Zenix SEC-300, 30 cm X 4.6 mm I.D., 3 µm particles (Z777087)
column temp. ambient
mobile phase 0.15 M potassium phosphate monobasic, pH 7.0 with potassium hydroxide
flow rate 0.35 ml/min
pressure 1350 psi (93 bar)
sample digested SO57 mAb, ~0.5 g/L
injection 1 µl
detector UV, 215 nm

Description

Analysis Note Proteolysis of antibodies can be useful for simplifying further analysis. This application demonstrates separation of the two fragments, F(ab′)2 and scFc from the intact IgG, after partial digestion with IdeS protease.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information Zenix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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