HPLC Analysis of a Peptide Drug on Antibodix® WCX-NP10, Gradient Optimization to Separate Impurity

HPLC Analysis of a Peptide Drug on Antibodix® WCX-NP10, Gradient Optimization to Separate Impurity

Conditions

column Antibodix WCX-NP10, 25 cm x 4.6 mm I.D., 10 µm particles (Z777272)
column temp. ambient
mobile phase [A] 3 mM potassium phosphate, pH 5.0; [B] 20 mM potassium phosphate, pH 7.5
gradient (A) 0% - 50% B (20 min); (B) 0% - 20% B (20 min), 20% B after 20 min
flow rate 1 mL/min
sample 2 mg/mL, proprietary peptide drug, pI 7-8
injection 5 µL

Description

Categories Analytical Chromatography, Peptides
Featured Industry Life Science and Biopharma
Pharmaceutical (small molecule)
Legal Information Antibodix is a registered trademark of Sepax Technologies
suitability application for HPLC

Materials

     
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