HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR

HPLC Analysis of a Procainamide-Labeled Dextran Ladder on BIOshell™ Glycan using HILIC-FLR

Conditions

column HPLC BIOshell Glycan Column, 15 cm x 2.1 mm I.D., 2.7 µm particles (50994-U)
mobile phase A: 50 mM ammonium formate (CAt. No. 17843) pH 4.4, [ammonium] = 50 mM, pH adjust with formic acid (Fluka 94318) B: Acetonitrile (Sigma-Aldrich 34851)
gradient 75% to 59% B in 75 min
flow rate 0.3 mL/min
sample Dextran Ladder (31417) after Procainamide Labeling Protocol
injection 10 µL
detector FLR (308 nm excitation, 359 emission)

Description

Analysis Note The dextran ladder is used as an external standard for the analysis of glycans by normal phase HPLC after fluorescent labeling. When analyzed on the BIOshell Glycan HPLC Column, this standard gives a characteristic ladder profile from monomeric glucose to approximately a 20-mers of glucose oligosaccharide. This ladder provides calibration reference points that can aide in identifying more complex glycans based upon retention characteristics.
Categories Analytical Chromatography, Proteins, Peptides
Featured Industry Life Science and Biopharma
Legal Information BIOshell is a trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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