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HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18

HPLC Analysis of Acetaminophen, Caffeine, Chlorpheniramine, Ibuprofen, Naproxen, Phenylephrine, and Pseudoephedrine on Ascentis® Express C18

Conditions

column Ascentis Express C18, 5 cm x 2.1 mm I.D., 2.7 µm particles (53822-U)
column temp. 35 C
mobile phase [A] 5 mM ammonium phosphate monobasic, pH 2.0 with phosphoric acid: acetonitrile (98:2); [B] acetonitrile;
gradient 0 to 68% B in 3 min; held at 68% B for 2 min
flow rate 0.4 ml/min
sample 100 µg/mL in 95:5, water: methanol
injection 1 µl
detector UV, 210 nm

Description

Analysis Note This application demonstrates the suitability of Ascentis Express C18 for the efficient separation of various over the counter drugs.
Categories Analytical Chromatography, Analgesics
Featured Industry Pharmaceutical (small molecule)
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC

Materials

     
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