HPLC Analysis of Acrylamide in Fried Potato Chips on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and DSC-18

HPLC Analysis of Acrylamide in Fried Potato Chips on Discovery® HS F5 after SPE using Discovery® DSC-MCAX and DSC-18

Conditions

sample preparation SPE (Solid Phase Extraction)
sample/matrix Finely grind 2.0 g of potato chips, place in 20 mL vial and add 10 mL water. Mix using vortex until mixture forms a thick paste. (Place sample in centrifuge tubes and centrifuge at 16.1 rcf for 5 min. Extract aqueous portion of sample leaving oil layer and solids.)
SPE tube/cartridge Discovery DSC-MCAX, 300 mg/3 mL (52784-U)
SPE tube/cartridge Discovery DSC-18, 1g/6mL (52606-U)
condition connect a Discovery DSC-MCAX SPE tube to the top of a Discovery DSC-18 SPE tube using an SPE tube adapter, pass 1 mL methanol, then 1 mL DI water through both SPE tubes until the tubes are dry
sample addition 1 mL of aqueous extract
washing 1 mL DI water
elution removing upper MCAX SPE tube, pass 2 mL methanol through lower DSC-18 SPE tube
eluate post-treatment evaporate to dryness with nitrogen at 30 C, reconstitute in 0.5 mL DI water
column Discovery HS F5, 15 cm x 4.6 mm I.D., 3 µm particles (567507-U)
column temp. 35 C
mobile phase 100% ultra-pure water
injection 5 µl
detector ESI+

Description

Categories Analytical Chromatography, SPE Applications, Food & Beverage Analysis, Flavors/fragrances, Food Industry Applications, Processing/Packaging Contaminants, Acrylamide, Applications (Chromatograms)
Featured Industry Food and Beverages
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
Other Notes This is an application, not a product for sale.
See "Related Products" below to find associated products for sale.
For more information, email: Supelco Technical Service
suitability application for SPE, application for HPLC

Materials

     
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