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HPLC Analysis of Aflatoxin Analogs on Ascentis® C18

HPLC Analysis of Aflatoxin Analogs on Ascentis® C18


column Ascentis C18, 15 cm x 4.6 mm I.D., 3 µm particles (581322-U)
column temp. 30 C or 50 C
mobile phase [A] acetonitrile: [B] methanol: [C] water; (20:20:60, A:B:C)
flow rate 1.0 ml/min
sample Aflatoxin Standard Mix, diluted 1:1 with water
injection 20 µl
detector fluorescence, 360 nm excitation, 455 nm emission


Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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