HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea

HPLC Analysis of Aflatoxins in Pistachios on Titan™ C18 following SPE using Supel™ Tox AflaZea

Conditions

sample/matrix pistachios
SPE tube/cartridge SupelTox AflaZea, 6mL (55314-U)
sample addition directly load 2 mL extract onto SPE tube
elution pull sample through the SPE tube by vacuum at a rate of 8 ml/min
eluate post-treatment combine 200 µl eluate and 300 µl of water:trifluoroacetic acid:acetic acid (70:20:10 ) derivatization reagent, (heat at 65C for 25 min, add 500 µl of water to derivatized sample and vortex 30 sec)
column Titan C18, 10 cm x 2.1 mm I.D., 1.9 µm particles (577124-U)
column temp. 35 C
mobile phase (A) water; (B) acetonitrile
gradient 10% to 30% B in 3 min; to 100% B in 2 min; held at 100% B for 2 min; to 10% B in 0.5 min; held at 10% B for 2.5 min
flow rate 0.4 ml/min
sample In pistachios G1, B1 (15 ppb), G2, B2 (3.75 ppb)
injection 10 µl
detector fluorescence, ex 365 nm, em 450 nm

Description

Analysis Note The presence and levels of aflatoxins in pistachio nuts are regulated. This application shows the analysis at the concentration levels of 15 ppb. The use of UHPLC-Fluoresence results in sensitive analysis while using Solid Phase Extraction (SPE) cleanup.
Categories Analytical Chromatography, Mycotoxins
Featured Industry Food and Beverages
Legal Information Supel is a trademark of Sigma-Aldrich Co. LLC, Titan is a trademark of Sigma-Aldrich Co. LLC
Other Notes extract 25 g of ground pistachios into 100 mL of 86:14 acetonitrile:water by shaking for 1 hour
suitability application for SPE, application for HPLC

Materials

     
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