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HPLC Analysis of Alkaloids on Discovery® Zr-PBD

HPLC Analysis of Alkaloids on Discovery® Zr-PBD


column Discovery Zr-PBD, 15 cm x 4.6 mm I.D., 5 µm particles (65723-U)
column temp. 65 C
mobile phase [A] 20 mM potassium phosphate (pH 12): [B] acetonitrile; (90:10, A:B)
flow rate 2.35 ml/min
injection 10 µl each compound 50 µg/mL
detector UV, 220 nm


Categories Analytical Chromatography, Pharmaceutical Analysis, Alkaloids
Featured Industry Pharmaceutical (small molecule)
Vitamins, Nutraceuticals, and Natural Products
Legal Information Discovery is a registered trademark of Sigma-Aldrich Co. LLC
suitability application for HPLC


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